Abstract
The gene complex encoding all determinants of the biosynthesis pathway of the capsule of group B meningococci (cps) has been cloned in Escherichia coli. A 24-kilobase large chromosomal fragment is necessary for capsule expression on the E. coli surface. By transposon and deletion mutagenesis, two separate steps in transport of the polysaccharide from the cytoplasm to the periplasm and further to the cell surface became evident. Mutants were also isolated that accumulate soluble poly(sialic acid) in the cytoplasm. The cloned cps complex conferred to E. coli strain GC6 sensitivity for E. coli K1-specific phages; phage sensitivity was enhanced in two distinct classes of cps mutants. Southern blot experiments revealed homology to some or all other Neisseria meningitidis capsular types and other Neisseria species, depending on the fragment of the cps complex used as probe.
MeSH Terms
Cell Membrane/analysis
Cloning, Molecular
DNA, Recombinant/metabolism
Escherichia coli/genetics
Genes
Genes, Bacterial
Multigene Family
Mutation
Neisseria meningitidis/genetics
Plasmids
Polysaccharides, Bacterial/genetics
Restriction Mapping
Sialic Acids/analysis,genetics
Chemicals
DNA, Recombinant
Polysaccharides, Bacterial
Sialic Acids
polysialic acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Frosch M
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Federal Republic of Germany.
Weisgerber C
Meyer T F
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