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PMID: 24941006 Published · epublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparative proteome analysis of cryopreserved flagella and head plasma membrane proteins from sea bream spermatozoa: effect of antifreeze proteins.

PloS one ·Vol. 9 ·No. 6 ·2014-00-00 ·Pages e99992

Zilli L, Beirão J, Schiavone R, Herraez MP, Gnoni A, Vilella S

Abstract

Cryopreservation induces injuries to fish spermatozoa that in turn affect sperm quality in terms of fertilization ability, motility, DNA and protein integrity and larval survival. To reduce the loss of sperm quality due to freezing-thawing, it is necessary to improve these procedures. In the present study we investigated the ability of two antifreeze proteins (AFPI and AFPIII) to reduce the loss of quality of sea bream spermatozoa due to cryopreservation. To do so, we compared viability, motility, straight-line velocity and curvilinear velocity of fresh and (AFPs)-cryopreserved spermatozoa. AFPIII addition to cryopreservation medium improved viability, motility and straight-line velocity with respect to DMSO or DMSO plus AFPI. To clarify the molecular mechanism(s) underlying these findings, the protein profile of two different cryopreserved sperm domains, flagella and head plasma membranes, was analysed. The protein profiles differed between fresh and frozen-thawed semen and results of the image analysis demonstrated that, after cryopreservation, out of 270 proteins 12 were decreased and 7 were increased in isolated flagella, and out of 150 proteins 6 showed a significant decrease and 4 showed a significant increase in head membranes. Mass spectrometry analysis identified 6 proteins (4 from isolated flagella and 2 present both in flagella and head plasma membranes) within the protein spots affected by the freezing-thawing procedure. 3 out of 4 proteins from isolated flagella were involved in the sperm bioenergetic system. Our results indicate that the ability of AFPIII to protect sea bream sperm quality can be, at least in part, ascribed to reducing changes in the sperm protein profile occurring during the freezing-thawing procedure. Our results clearly demonstrated that AFPIII addition to cryopreservation medium improved the protection against freezing respect to DMSO or DMSO plus AFPI. In addition we propose specific proteins of spermatozoa as markers related to the procedures of fish sperm cryopreservation.

MeSH Terms
Animals Antifreeze Proteins/pharmacology Cell Membrane/drug effects,metabolism Cell Survival/drug effects Cryopreservation Cryoprotective Agents/pharmacology Electrophoresis, Gel, Two-Dimensional Fertilization/drug effects Fish Proteins/metabolism Flagella/metabolism Male Mass Spectrometry Membrane Proteins/metabolism Proteome/metabolism Proteomics/methods Sea Bream/metabolism Sperm Head/drug effects,metabolism Sperm Motility/drug effects
Chemicals
Antifreeze Proteins Cryoprotective Agents Fish Proteins Membrane Proteins Proteome
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zilli Loredana
Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
Beirão José
Department of Molecular Biology, University of León, León, Spain.
Schiavone Roberta
Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
Herraez Maria Paz
Department of Molecular Biology, University of León, León, Spain.
Gnoni Antonio
Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, Italy.
Vilella Sebastiano
Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0099992
Published
2014-00-00
Epub
2014-00-18
Pages
e99992
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC4062426
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0099992 PMC Full Text View journal details
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