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PMID: 2501219 Published · ppublish English Journal Article

Selective inhibition of cytokine-induced lysozyme activity by tetanus toxin in the GG2EE macrophage cell line.

Infection and immunity ·Vol. 57 ·No. 8 ·1989-08-00 ·Pages 2452-6

Pitzurra L, Marconi P, Bistoni F, Blasi E

Abstract

This study was designed to evaluate the effects of tetanus toxin (TT) on lysozyme (LZM) activity by the GG2EE macrophage cell line. GG2EE cells spontaneously produced low amounts of LZM, which were mostly secreted into the culture medium. Upon treatment with various cytokines, GG2EE cells exhibited altered LZM activity. In particular, exposure of GG2EE cells to alpha/beta interferon (IFN-alpha/beta) reduced LZM activity, as opposed to treatment with gamma interferon (IFN-gamma) or colony-stimulating factor 1, which potentiated LZM activity. Spontaneous LZM activity of GG2EE cells was not susceptible to TT action; in contrast, when IFN-gamma- or colony-stimulating factor 1-susceptible cells were treated with TT, a significant reduction on LZM activity was observed. The TT inhibitory effect was dose dependent and manifested only after a 6-h incubation of GG2EE cells with TT. Treatment of GG2EE cells with heat-inactivated TT as well as Ibc- and B-IIb-TT-derived fragments was found to be ineffective, while pretreatment with B-IIb- but not with Ibc-TT-derived fragment abrogated the TT effect. Overall, these data indicate the existence of a specific TT-GG2EE cell interaction, leading to selective inhibition of cytokine-induced LZM activity.

MeSH Terms
Animals Binding, Competitive Biological Factors/pharmacology Cell Line Colony-Stimulating Factors/pharmacology Cytokines Interferon Type I/pharmacology Interferon-gamma/pharmacology Kinetics Macrophages/enzymology Mice Molecular Weight Muramidase/antagonists & inhibitors Peptide Fragments/pharmacology Tetanus Toxin/antagonists & inhibitors,pharmacology
Chemicals
Biological Factors Colony-Stimulating Factors Cytokines Interferon Type I Peptide Fragments Tetanus Toxin Interferon-gamma Muramidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pitzurra L
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Marconi P
Bistoni F
Blasi E
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.8.2452-2456.1989
Published
1989-08-00
Pages
2452-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313469
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.8.2452-2456.1989 PMC Full Text View journal details
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