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PMID: 2501794 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antibiotic proteins of human polymorphonuclear leukocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 14 ·1989-07-00 ·Pages 5610-4

Gabay JE, Scott RW, Campanelli D, Griffith J, Wilde C, Marra MN, Seeger M, Nathan CF

Abstract

Nine polypeptide peaks with antibiotic activity were resolved from human polymorphonuclear leukocyte azurophil granule membranes. All but 1 of the 12 constituent polypeptides were identified by N-terminal sequence analysis. Near quantitative recovery of protein and activity permitted an assessment of the contribution of each species to the overall respiratory-burst-independent antimicrobial capacity of the cell. Three uncharacterized polypeptides were discovered, including two broad-spectrum antibiotics. One of these, a defensin that we have designated human neutrophil antimicrobial peptide 4, was more potent than previously described defensins but represented less than 1% of the total protein. The other, named azurocidin, was abundant and comparable to bactericidal permeability-increasing factor in its contribution to the killing of Escherichia coli.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/blood,isolation & purification,pharmacology Blood Proteins/isolation & purification Candida albicans/drug effects Chromatography, High Pressure Liquid Enterococcus faecalis/drug effects Escherichia coli/drug effects Humans Microbial Sensitivity Tests Molecular Sequence Data Molecular Weight Neutrophils/analysis
Chemicals
Anti-Bacterial Agents Blood Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gabay J E
Beatrice and Samuel A. Seaver Laboratory, Department of Medicine, Cornell University Medical College, New York, NY 10021.
Scott R W
Campanelli D
Griffith J
Wilde C
Marra M N
Seeger M
Nathan C F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.14.5610
Published
1989-07-00
Pages
5610-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297672
Subset
IM
Grants
NIAID NIH HHS · AI 23807 · United States
NCI NIH HHS · CA 43610 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.86.14.5610 PMC Full Text View journal details
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