Home LiteratureArticle Details
PMID: 2504501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of the Escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi.

Current genetics ·Vol. 15 ·No. 3 ·1989-03-00 ·Pages 177-80

Roberts IN, Oliver RP, Punt PJ, van den Hondel CA

Abstract

A chimaeric beta-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Co-transformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke] resulted in the expression of beta-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi.

MeSH Terms
Aspergillus nidulans/genetics Aspergillus niger/genetics Cladosporium/genetics Culture Media Escherichia coli/genetics Gene Expression Regulation Genetic Vectors Glucuronidase/genetics,metabolism Plasmids Transformation, Genetic beta-Galactosidase/metabolism
Chemicals
Culture Media beta-Galactosidase Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Roberts I N
Norwich Molecular Plant Pathology Group, School of Biological Sciences, University of East Anglia, UK.
Oliver R P
Punt P J
van den Hondel C A
References (14)
14 references, click to expand
  1. Transformation of Aspergillus oryzae using the A. niger pyrG gene.
    Mol Gen Genet. 1987 Dec;210(3):460-1 PMID: 3481025
  2. Identification of plant-induced genes of the bacterial pathogen Xanthomonas campestris pathovar campestris using a promoter-probe plasmid.
    EMBO J. 1987 Jan;6(1):23-8 PMID: 15981331
  3. A system for the analysis of expression signals in Aspergillus.
    Gene. 1986;48(2-3):211-7 PMID: 3549463
  4. Expression of chimeric genes in Caenorhabditis elegans.
    J Mol Biol. 1987 Jan 5;193(1):41-6 PMID: 3295256
  5. Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.
    Mol Gen Genet. 1987 Jan;206(1):71-5 PMID: 3472035
  6. Expression analysis vectors for Aspergillus niger.
    Nucleic Acids Res. 1988 Sep 26;16(18):9052 PMID: 3174446
  7. beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51 PMID: 3534890
  8. The Rhizobium leguminosarum nodulation gene nodF encodes a polypeptide similar to acyl-carrier protein and is regulated by nodD plus a factor in pea root exudate.
    EMBO J. 1986 Apr;5(4):647-52 PMID: 16453679
  9. Cotransformation of Aspergillus nidulans: a tool for replacing fungal genes.
    Mol Gen Genet. 1987 Aug;209(1):71-7 PMID: 3312958
  10. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  11. Expression of an Escherichia coli beta-galactosidase fusion gene in Aspergillus nidulans.
    Gene. 1985;40(1):99-106 PMID: 3005133
  12. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.
    Gene. 1987;56(1):117-24 PMID: 2824287
  13. Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene.
    Gene. 1988;62(1):127-34 PMID: 3131191
  14. Characterisation of the 5'-leader sequence of tobacco mosaic virus RNA as a general enhancer of translation in vitro.
    Gene. 1987;60(2-3):217-25 PMID: 2832252
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00435503
Published
1989-03-00
Pages
177-80
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00435503 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2