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PMID: 2504726 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.

The Journal of cell biology ·Vol. 109 ·No. 3 ·1989-09-00 ·Pages 1015-22

Bacon RA, Salminen A, Ruohola H, Novick P, Ferro-Novick S

Abstract

The YPT1 gene encodes a raslike, GTP-binding protein that is essential for growth of yeast cells. We show here that mutations in the ypt1 gene disrupt transport of carboxypeptidase Y to the vacuole in vivo and transport of pro-alpha-factor to a site of extensive glycosylation in the Golgi apparatus in vitro. Two different ypt1 mutations result in loss of function of the Golgi complex without affecting the activity of the endoplasmic reticulum or soluble components required for in vitro transport. The function of the mutant Golgi apparatus can be restored by preincubation with wild-type cytosol. The transport defect observed in vitro cannot be overcome by addition of Ca++ to the reaction mixture. We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus.

MeSH Terms
Alleles Calcium/metabolism Carboxypeptidases/genetics Cathepsin A Fungal Proteins/genetics GTP-Binding Proteins/genetics,physiology Genes Genes, Fungal Glycosylation Golgi Apparatus/physiology Mutation Protein Precursors/genetics Protein Processing, Post-Translational Proteins/genetics Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Species Specificity Vacuoles/metabolism rab GTP-Binding Proteins
Chemicals
Fungal Proteins MF(ALPHA)1 protein, S cerevisiae Protein Precursors Proteins Saccharomyces cerevisiae Proteins Carboxypeptidases Cathepsin A PRC1 protein, S cerevisiae serine carboxypeptidase GTP-Binding Proteins YPT1 protein, S cerevisiae rab GTP-Binding Proteins Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bacon R A
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
Salminen A
Ruohola H
Novick P
Ferro-Novick S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.3.1015
Published
1989-09-00
Pages
1015-22
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115776
Subset
IM
Grants
NCI NIH HHS · CA 46128 · United States
NIGMS NIH HHS · GM35370 · United States
External Links
PubMed source DOI: 10.1083/jcb.109.3.1015 PMC Full Text View journal details
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