Home LiteratureArticle Details
PMID: 25048493 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Histone acetylation level and histone acetyltransferase/deacetylase activity in ejaculated sperm from normozoospermic men.

Yonsei medical journal ·Vol. 55 ·No. 5 ·2014-09-00 ·Pages 1333-40

Kim JH, Jee BC, Lee JM, Suh CS, Kim SH

Abstract

The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commercially available kits. Nuclear DNA integrity was also measured by TUNEL assay. The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationship with HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r=-0.53, p<0.01). Our works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI.

Keywords
Acetylation acetyltransferase deacetylase histone sperm
MeSH Terms
Acetylation Adult DNA Fragmentation Epigenesis, Genetic Histone Acetyltransferases/metabolism Histones/metabolism Humans Immunohistochemistry Male Middle Aged Protein Processing, Post-Translational Semen Analysis Spermatozoa/metabolism
Chemicals
Histones Histone Acetyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kim Jee Hyun
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea. ; Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
Jee Byung Chul
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea. [email protected] ; Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
Lee Jang Mi
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.
Suh Chang Suk
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea. ; Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
Kim Seok Hyun
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. ; Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
References (36)
36 references, click to expand
  1. Effect of cigarette smoking on antioxidant levels and presence of leukocytospermia in infertile men: a prospective study.
    Fertil Steril. 2008 Aug;90(2):278-83 PMID: 18462724
  2. Covalent modifications of histones: expression from chromatin templates.
    Curr Opin Genet Dev. 1998 Apr;8(2):173-8 PMID: 9610407
  3. Contributions of spermatozoa to embryogenesis: assays to evaluate their genetic and epigenetic fitness.
    Reprod Biomed Online. 2008 Apr;16(4):474-84 PMID: 18413055
  4. Paternal contribution: new insights and future challenges.
    Nat Rev Genet. 2005 Aug;6(8):633-42 PMID: 16136654
  5. Presence of histone H3 acetylated at lysine 9 in male germ cells and its distribution pattern in the genome of human spermatozoa.
    Reprod Fertil Dev. 2011;23(8):997-1011 PMID: 22127005
  6. Expression of hyperacetylated histone H4 during normal and impaired human spermatogenesis.
    Andrologia. 2002 Dec;34(6):384-90 PMID: 12472623
  7. The effect of epigenetic sperm abnormalities on early embryogenesis.
    Asian J Androl. 2006 Mar;8(2):131-42 PMID: 16491264
  8. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis.
    Hum Reprod. 2012 Oct;27(10):2908-17 PMID: 22791753
  9. Sperm nuclear DNA fragmentation and chromatin structure in one-day-old ejaculated sperm.
    Clin Exp Reprod Med. 2011 Jun;38(2):82-6 PMID: 22384423
  10. Sequential expression of nucleoproteins during rat spermiogenesis.
    Exp Cell Res. 1996 Jun 15;225(2):374-81 PMID: 8660926
  11. Prognostic markers for competent human spermatozoa: fertilizing capacity and contribution to the embryo.
    Int J Androl. 2011 Dec;34(6 Pt 1):513-27 PMID: 21128979
  12. Deacetylase enzymes: biological functions and the use of small-molecule inhibitors.
    Chem Biol. 2002 Jan;9(1):3-16 PMID: 11841934
  13. Misregulation of histone acetylation in Sertoli cell-only syndrome and testicular cancer.
    Mol Hum Reprod. 2003 Dec;9(12):757-63 PMID: 14614037
  14. Histone acetylation and control of gene expression.
    J Cell Sci. 1991 May;99 ( Pt 1):13-20 PMID: 1757496
  15. Isolation of four core histones from human sperm chromatin representing a minor subset of somatic histones.
    J Biol Chem. 1990 Nov 25;265(33):20662-6 PMID: 2243112
  16. Histone deacetylases (HDACs): characterization of the classical HDAC family.
    Biochem J. 2003 Mar 15;370(Pt 3):737-49 PMID: 12429021
  17. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis.
    Fertil Steril. 2010 Mar 1;93(4):1027-36 PMID: 20080235
  18. Regulated hyperacetylation of core histones during mouse spermatogenesis: involvement of histone deacetylases.
    Eur J Cell Biol. 2000 Dec;79(12):950-60 PMID: 11152286
  19. Paternal effects on cell division in the human preimplantation embryo.
    Reprod Biomed Online. 2005 Mar;10(3):370-5 PMID: 15820045
  20. Genomic DNA methylation: the mark and its mediators.
    Trends Biochem Sci. 2006 Feb;31(2):89-97 PMID: 16403636
  21. Epigenetic reprogramming of the male genome during gametogenesis and in the zygote.
    Reprod Biomed Online. 2008 Apr;16(4):492-503 PMID: 18413057
  22. Role of covalent modifications of histones in regulating gene expression.
    Gene. 1999 Nov 15;240(1):1-12 PMID: 10564807
  23. Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation.
    Hum Reprod. 2004 Mar;19(3):611-5 PMID: 14998960
  24. Semen quality of male smokers and nonsmokers in infertile couples.
    Fertil Steril. 2003 Feb;79(2):287-91 PMID: 12568836
  25. Smoking and infertility: a committee opinion.
    Fertil Steril. 2012 Dec;98(6):1400-6 PMID: 22959451
  26. Inadequate histone deacetylation during oocyte meiosis causes aneuploidy and embryo death in mice.
    Proc Natl Acad Sci U S A. 2006 May 9;103(19):7339-44 PMID: 16651529
  27. Relationship between semen quality and tobacco chewing in men undergoing infertility evaluation.
    Fertil Steril. 2005 Sep;84(3):649-53 PMID: 16169398
  28. Paternal contributions to the mammalian zygote: fertilization after sperm-egg fusion.
    Int Rev Cytol. 2000;195:1-65 PMID: 10603574
  29. Distinctive chromatin in human sperm packages genes for embryo development.
    Nature. 2009 Jul 23;460(7254):473-8 PMID: 19525931
  30. Differential histone modifications mark mouse imprinting control regions during spermatogenesis.
    EMBO J. 2007 Feb 7;26(3):720-9 PMID: 17255950
  31. DNMT1 and HDAC1 gene expression in impaired spermatogenesis and testicular cancer.
    Histochem Cell Biol. 2007 Feb;127(2):175-81 PMID: 16960727
  32. Mature human spermatozoa do not transcribe novel RNA.
    Andrologia. 2005 Jun;37(2-3):69-71 PMID: 16026427
  33. The common marmoset (Callithrix jacchus) as a model for histone and protamine expression during human spermatogenesis.
    Hum Reprod. 2009 Mar;24(3):536-45 PMID: 19056771
  34. Epigenetics and its role in male infertility.
    J Assist Reprod Genet. 2012 Mar;29(3):213-23 PMID: 22290605
  35. In vivo effects of histone-deacetylase inhibitor trichostatin-A on murine spermatogenesis.
    J Androl. 2004 Sep-Oct;25(5):811-8 PMID: 15292114
  36. Histone deacetylase 1 (HDAC1) regulates histone acetylation, development, and gene expression in preimplantation mouse embryos.
    Dev Biol. 2008 Jul 1;319(1):110-20 PMID: 18501342
Article Info
Journal
Yonsei medical journal
Abbr.
Yonsei Med J
ISSN
1976-2437
DOI
10.3349/ymj.2014.55.5.1333
Email
[email protected]
Published
2014-09-00
Pages
1333-40
Language
English
Region
Korea (South)
NLM ID
0414003
PMCID
PMC4108820
Subset
IM
External Links
PubMed source DOI: 10.3349/ymj.2014.55.5.1333 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2