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PMID: 2508088 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 19 ·1989-10-00 ·Pages 7407-9

Strathmann M, Wilkie TM, Simon MI

Abstract

Biochemical analysis has revealed a number of guanine nucleotide-binding regulatory proteins (G proteins) that mediate signal transduction in mammalian systems. Characterization of their cDNAs uncovered a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of diversity of the G protein family, we used the polymerase chain reaction to detect additional gene products in mouse brain and spermatid RNA that share these conserved regions. Sequences corresponding to six of the eight known G protein alpha subunits were obtained. In addition, we found sequences corresponding to five newly discovered alpha subunits. Our results suggest that the complexity of the G protein family is much greater than previously suspected.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain/metabolism GTP-Binding Proteins/genetics Genes Macromolecular Substances Male Mice Molecular Sequence Data Multigene Family Oligonucleotide Probes Polymerase Chain Reaction Sequence Homology, Nucleic Acid Spermatids/metabolism
Chemicals
Macromolecular Substances Oligonucleotide Probes GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Strathmann M
Division of Biology, California Institute of Technology, Pasadena 91125.
Wilkie T M
Simon M I
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.19.7407
Published
1989-10-00
Pages
7407-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298071
Subset
IM
Databases
GENBANK
M26739, M26740, M26741, M26742, M26743
External Links
PubMed source DOI: 10.1073/pnas.86.19.7407 PMC Full Text View journal details
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