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PMID: 2509432 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 171 ·No. 11 ·1989-11-00 ·Pages 6259-64

Klebl F, Tanner W

Abstract

A major protein of Saccharomyces cerevisiae cell walls is a 29-kilodalton glycoprotein which shows lectinlike binding to beta-1,3-glucan and chitin. It was solubilized by heating isolated cell walls at 90 degrees C and purified to homogeneity by running two high-pressure liquid chromatography columns. With the sequence information of the N terminus and seven peptides, two oligonucleotides were synthesized and the gene was cloned. Its sequence is similar to those of two plant beta-glucanases, and the protein was shown to possess beta-1,3-exoglucanase activity with laminarin as substrate. Haploid yeast cells contained one copy of the gene (BGL2). Gene disruption did not result in a phenotype.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Wall/enzymology Cloning, Molecular DNA, Fungal/genetics,isolation & purification Escherichia coli/genetics Genes, Fungal Glucan 1,3-beta-Glucosidase Glucosidases/genetics Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid beta-Glucosidase/genetics
Chemicals
DNA, Fungal Glucosidases beta-Glucosidase Glucan 1,3-beta-Glucosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klebl F
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Federal Republic of Germany.
Tanner W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.11.6259-6264.1989
Published
1989-11-00
Pages
6259-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210497
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.11.6259-6264.1989 PMC Full Text View journal details
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