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PMID: 2512297 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A galactosyltransferase from the fission yeast Schizosaccharomyces pombe.

The Journal of cell biology ·Vol. 109 ·No. 6 Pt 1 ·1989-12-00 ·Pages 2693-702

Chappell TG, Warren G

Abstract

A membrane-associated galactosyltransferase has been purified to homogeneity from the fission yeast, Schizosaccharomyces pombe. The enzyme has a molecular weight of 61,000 and is capable of transfering galactose from UDP-galactose (UDP-Gal) to a variety of mannose-based acceptors to form an alpha-1,2 galactosyl mannoside linkage. Immunofluorescence localization of the protein is consistent with the presence of the enzyme in the Golgi apparatus of S. pombe. This, together with the presence of terminal, alpha-linked galactose on the N-linked oligosaccharides of S. pombe secretory proteins, suggests that the galactosyltransferase is an enzyme involved in the processing of glycoproteins transported through the Golgi apparatus in fission yeast.

MeSH Terms
Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Cytosol/enzymology Edetic Acid/pharmacology Fluorescent Antibody Technique Galactosyltransferases/isolation & purification,metabolism Glycoproteins/genetics Golgi Apparatus/enzymology,ultrastructure Histocytochemistry Kinetics Manganese/pharmacology Microsomes/enzymology Molecular Weight Protein Processing, Post-Translational Saccharomycetales/enzymology Schizosaccharomyces/enzymology,ultrastructure Substrate Specificity
Chemicals
Glycoproteins Manganese Edetic Acid Galactosyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chappell T G
Imperial Cancer Research Fund, London, United Kingdom.
Warren G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.6.2693
Published
1989-12-00
Pages
2693-702
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115949
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.109.6.2693 PMC Full Text View journal details
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