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PMID: 2513331 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microvesicles of the neurohypophysis are biochemically related to small synaptic vesicles of presynaptic nerve terminals.

The Journal of cell biology ·Vol. 109 ·No. 6 Pt 2 ·1989-12-00 ·Pages 3425-33

Navone F, Di Gioia G, Jahn R, Browning M, Greengard P, De Camilli P

Abstract

Nerve endings of the posterior pituitary are densely populated by dense-core neurosecretory granules which are the storage sites for peptide neurohormones. In addition, they contain numerous clear microvesicles which are the same size as small synaptic vesicles of typical presynaptic nerve terminals. Several of the major proteins of small synaptic vesicles of presynaptic nerve terminals are present at high concentration in the posterior pituitary. We have now investigated the subcellular localization of such proteins. By immunogold electron microscopy carried out on bovine neurohypophysis we have found that three of these proteins, synapsin I, Protein III, and synaptophysin (protein p38) were concentrated on microvesicles but were not detectable in the membranes of neurosecretory granules. In addition, we have studied the distribution of the same proteins and of the synaptic vesicle protein p65 in subcellular fractions of bovine posterior pituitaries obtained by sucrose density centrifugation. We have found that the intrinsic membrane proteins synaptophysin and p65 had an identical distribution and were restricted to low density fractions of the gradient which contained numerous clear microvesicles with a size range the same as that of small synaptic vesicles. The peripheral membrane proteins synapsin I and Protein III exhibited a broader distribution extending into the denser part of the gradient. However, the amount of these proteins clearly declined in the fractions preceding the peak of neurosecretory granules. Our results suggest that microvesicles of the neurohypophysis are biochemically related to small synaptic vesicles of all other nerve terminals and argue against the hypothesis that such vesicles represent an endocytic byproduct of exocytosis of neurosecretory granules.

MeSH Terms
Animals Antibodies, Monoclonal Blotting, Western Cattle Centrifugation, Density Gradient Cytoplasmic Granules/analysis Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Immunoblotting Membrane Proteins/analysis Nerve Endings/metabolism Nerve Tissue Proteins/analysis Neuropeptides/analysis Pituitary Gland, Posterior/analysis,innervation,ultrastructure Rats Rats, Inbred Strains Subcellular Fractions/analysis Synapsins Synaptic Vesicles/analysis,ultrastructure Synaptophysin
Chemicals
Antibodies, Monoclonal Membrane Proteins Nerve Tissue Proteins Neuropeptides Synapsins Synaptophysin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Navone F
Consiglio Nazionale delle Ricerche Center of Cytopharmacology, University of Milano, Italy.
Di Gioia G
Jahn R
Browning M
Greengard P
De Camilli P
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44 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.6.3425
Published
1989-12-00
Pages
3425-33
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115912
Subset
IM
Grants
NIMH NIH HHS · MH39327 · United States
NINDS NIH HHS · NS21550 · United States
External Links
PubMed source DOI: 10.1083/jcb.109.6.3425 PMC Full Text View journal details
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