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PMID: 2521491 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Adherence of neutrophils to cultured human microvascular endothelial cells. Stimulation by chemotactic peptides and lipid mediators and dependence upon the Mac-1, LFA-1, p150,95 glycoprotein family.

The Journal of clinical investigation ·Vol. 83 ·No. 2 ·1989-02-00 ·Pages 637-46

Tonnesen MG, Anderson DC, Springer TA, Knedler A, Avdi N, Henson PM

Abstract

The process of neutrophil adhesion to and migration through the microvascular endothelium, an early event in the induction of the acute inflammatory response, has been attributed to the generation of extravascular chemoattractants. Although both chemotactic peptides and lipid mediators enhance neutrophil adherence in vitro and in vivo, the mechanism(s) involved in the interaction between circulating neutrophils and microvascular endothelial cells is still not completely understood. In a microtiter well adherence assay, the chemotactic peptides, FMLP and C5a, and the lipid mediators, leukotriene B4 (LTB4) and platelet activating factor (PAF), enhanced human neutrophil adherence to cultured human microvascular endothelial cells as well as to human umbilical vein endothelial cells in a dose-dependent manner with a rapid time course. This stimulated adhesive interaction between neutrophils and cultured human endothelial cells was dependent on the expression of the Mac-1, LFA-1, p150,95 glycoprotein family on the neutrophil surface since neutrophils from patients with leukocyte adhesion deficiency, lacking surface expression of the adhesive glycoproteins, exhibited markedly diminished adherence to human endothelial cells in response to stimulation with chemotactic factors compared to normal control neutrophils. All four mediators enhanced expression of the glycoprotein family on the surface of normal neutrophils as determined by flow cytofluorimetry using a monoclonal antibody (TS1/18) to the glycoprotein common beta subunit. In addition, TS1/18 inhibited up to 100% the adherence of normal neutrophils to endothelial cells stimulated by maximal concentrations of FMLP, C5a, LTB4, or PAF. Moreover, HL-60 cells, human promyelocytic leukemia cells, neither increased glycoprotein surface expression nor adherence in response to stimulation. Thus, peptide and lipid mediators of the acute inflammatory response appear to enhance adherence of circulating neutrophils to the microvascular endothelium by a mechanism dependent on expression of the Mac-1, LFA-1, p150,95 glycoprotein family on the neutrophil surface.

MeSH Terms
Antigens, Differentiation/pharmacology Cell Adhesion/drug effects Cells, Cultured Chemotactic Factors/pharmacology Endothelium, Vascular/cytology Humans Integrin alphaXbeta2 Leukemia, Myeloid, Acute/pathology Lipid Metabolism Lymphocyte Function-Associated Antigen-1 Membrane Glycoproteins/biosynthesis Microcirculation Neutrophils/cytology Receptors, Complement/metabolism Receptors, Complement 3b
Chemicals
Antigens, Differentiation Chemotactic Factors Integrin alphaXbeta2 Lymphocyte Function-Associated Antigen-1 Membrane Glycoproteins Receptors, Complement Receptors, Complement 3b
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tonnesen M G
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
Anderson D C
Springer T A
Knedler A
Avdi N
Henson P M
References (61)
61 references, click to expand
  1. Inherited deficiency of the Mac-1, LFA-1, p150,95 glycoprotein family and its molecular basis.
    J Exp Med. 1984 Dec 1;160(6):1901-18 PMID: 6096477
  2. Deficiency of a leukocyte surface glycoprotein (LFA-1) in two patients with Mo1 deficiency. Effects of cell activation on Mo1/LFA-1 surface expression in normal and deficient leukocytes.
    J Clin Invest. 1984 Oct;74(4):1291-300 PMID: 6237120
  3. Human leukemic models of myelomonocytic development: a review of the HL-60 and U937 cell lines.
    J Leukoc Biol. 1985 Apr;37(4):407-22 PMID: 3855947
  4. Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide.
    Am J Pathol. 1985 Apr;119(1):101-10 PMID: 2984939
  5. Abnormal adherence-related functions of neutrophils, monocytes, and Epstein-Barr virus-transformed B cells in a patient with C3bi receptor deficiency.
    Blood. 1985 Jun;65(6):1382-90 PMID: 2986743
  6. The role of neutrophil membrane glycoprotein GP-150 in neutrophil adherence to endothelium in vitro.
    Blood. 1985 Jul;66(1):167-78 PMID: 4005427
  7. The severe and moderate phenotypes of heritable Mac-1, LFA-1 deficiency: their quantitative definition and relation to leukocyte dysfunction and clinical features.
    J Infect Dis. 1985 Oct;152(4):668-89 PMID: 3900232
  8. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines.
    J Clin Invest. 1985 Nov;76(5):2003-11 PMID: 3877078
  9. p150,95, the third member of the Mac-1, LFA-1 human leukocyte adhesion glycoprotein family.
    J Immunol. 1986 Jan;136(1):240-5 PMID: 3510003
  10. Monoclonal antibody-defined functional epitopes on the adhesion-promoting glycoprotein complex (CDw18) of human neutrophils.
    Blood. 1986 Apr;67(4):1007-13 PMID: 2420394
  11. Neutrophil-mediated injury to endothelial cells. Enhancement by endotoxin and essential role of neutrophil elastase.
    J Clin Invest. 1986 Apr;77(4):1233-43 PMID: 3485659
  12. An endothelial cell surface factor(s) induced in vitro by lipopolysaccharide, interleukin 1, and tumor necrosis factor-alpha increases neutrophil adherence by a CDw18-dependent mechanism.
    J Immunol. 1986 Jun 15;136(12):4548-53 PMID: 3486903
  13. Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.
    J Immunol. 1986 Jul 1;137(1):15-27 PMID: 3519773
  14. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    J Immunol. 1986 Nov 1;137(9):2891-900 PMID: 2428876
  15. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies.
    J Immunol. 1986 Nov 15;137(10):3259-63 PMID: 2430017
  16. A monoclonal antibody to the membrane glycoprotein complex CD18 inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo.
    Blood. 1987 Jan;69(1):338-40 PMID: 3539230
  17. Either exogenous or endogenous fibronectin can promote adherence of human endothelial cells.
    J Cell Sci. 1986 Jun;82:263-80 PMID: 3539950
  18. Expression of leukocyte adherence-related glycoproteins during differentiation of HL-60 promyelocytic leukemia cells.
    J Immunol. 1987 Jan 15;138(2):513-9 PMID: 2947951
  19. Association between gelatinase release and increased plasma membrane expression of the Mo1 glycoprotein.
    Blood. 1987 Feb;69(2):605-10 PMID: 3026524
  20. Human dermal microvascular endothelial cells behave like umbilical vein endothelial cells in T-cell adhesion studies.
    J Invest Dermatol. 1987 Mar;88(3):340-4 PMID: 3102624
  21. Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins.
    Annu Rev Med. 1987;38:175-94 PMID: 3555290
  22. Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum.
    In Vitro Cell Dev Biol. 1987 Jul;23(7):481-91 PMID: 3301790
  23. Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface.
    J Clin Invest. 1987 Aug;80(2):535-44 PMID: 3038962
  24. Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture.
    J Immunol. 1987 Sep 1;139(5):1557-62 PMID: 3497975
  25. Aggregation of complement receptors on human neutrophils in the absence of ligand.
    J Cell Biol. 1987 Sep;105(3):1137-45 PMID: 2958480
  26. In vivo inhibition of neutrophil function in the rabbit using monoclonal antibody to CD18.
    J Immunol. 1987 Dec 15;139(12):4174-7 PMID: 3693901
  27. Neutrophil adherence to human endothelium in vitro occurs by CDw18 (Mo1, MAC-1/LFA-1/GP 150,95) glycoprotein-dependent and -independent mechanisms.
    J Clin Invest. 1988 Feb;81(2):531-7 PMID: 2828429
  28. Increased surface expression of CD11b/CD18 (Mac-1) is not required for stimulated neutrophil adherence to cultured endothelium.
    J Clin Invest. 1988 Mar;81(3):676-82 PMID: 3278004
  29. Partial characterization of human C5a anaphylatoxin. I. Chemical description of the carbohydrate and polypeptide prtions of human C5a.
    J Immunol. 1976 Nov;117(5 Pt 1):1688-94 PMID: 1002996
  30. Culture of vascular endothelium.
    Prog Hemost Thromb. 1976;3:1-28 PMID: 181786
  31. Up-regulation of the iC3b receptor (CR3) is neither necessary nor sufficient to promote neutrophil aggregation.
    J Clin Invest. 1988 Aug;82(2):495-501 PMID: 2841354
  32. Neutropenia induced by systemic infusion of chemotactic factors.
    J Immunol. 1977 May;118(5):1586-9 PMID: 858915
  33. In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions. Studies based on the acute neutropenia of filtration leukophoresis.
    J Exp Med. 1977 Sep 1;146(3):641-52 PMID: 894188
  34. The adhesive interaction between polymorphonuclear leukocytes and endothelial cells in vitro.
    Cell. 1978 Jun;14(2):423-8 PMID: 667949
  35. Intracellular control of human neutrophil secretion. I. C5a-induced stimulus-specific desensitization and the effects of cytochalasin B.
    J Immunol. 1978 Sep;121(3):851-5 PMID: 211165
  36. Normal functional characteristics of cultured human promyelocytic leukemia cells (HL-60) after induction of differentiation by dimethylsulfoxide.
    J Exp Med. 1979 Apr 1;149(4):969-74 PMID: 219131
  37. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia.
    Blood. 1979 Sep;54(3):713-33 PMID: 288488
  38. The effects of chemotactic factors on the adhesiveness of rabbit neutrophil granulocytes.
    Exp Cell Res. 1979 Aug;122(1):169-77 PMID: 477754
  39. Functional changes in human leukemic cell line HL-60. A model for myeloid differentiation.
    J Cell Biol. 1979 Aug;82(2):315-22 PMID: 225336
  40. Biologial effects of the human complement fragments C5a and C5ades Arg on neutrophil function.
    Immunopharmacology. 1980 Jun;2(3):201-19 PMID: 6254906
  41. Adhesion of leukocytes to endothelium: roles of divalent cations, surface charge, chemotactic agents and substrate.
    J Cell Sci. 1980 Oct;45:73-86 PMID: 7462350
  42. Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: in vivo effects with relevance to the acute inflammatory response.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3887-91 PMID: 6267608
  43. Leukotriene B4 stimulates polymorphonuclear leukocyte adhesion to cultured vascular endothelial cells.
    J Clin Invest. 1984 Oct;74(4):1552-5 PMID: 6090507
  44. Human neutrophils increase expression of C3bi as well as C3b receptors upon activation.
    J Clin Invest. 1984 Nov;74(5):1566-71 PMID: 6209300
  45. Neutrophil-endothelial cell interactions. Modulation of neutrophil adhesiveness induced by complement fragments C5a and C5a des arg and formyl-methionyl-leucyl-phenylalanine in vitro.
    J Clin Invest. 1984 Nov;74(5):1581-92 PMID: 6501563
  46. Microvascular effects of anaphylatoxins C3a and C5a.
    J Immunol. 1985 Feb;134(2):1115-9 PMID: 3871207
  47. A monoclonal antibody inhibiting human neutrophil chemotaxis and degranulation.
    J Immunol. 1981 Oct;127(4):1355-60 PMID: 6268706
  48. Metabolic, membrane, and functional responses of human polymorphonuclear leukocytes to platelet-activating factor.
    Blood. 1982 Jun;59(6):1259-66 PMID: 6282362
  49. Studies on the interaction between GP-18-0-deficient neutrophils and vascular endothelium.
    Blood. 1982 Jul;60(1):160-5 PMID: 7044449
  50. Pulmonary microvascular alterations and injury induced by complement fragments: synergistic effect of complement activation, neutrophil sequestration, and prostaglandins.
    Ann N Y Acad Sci. 1982;384:287-300 PMID: 6953825
  51. The microvasculature in inflammation.
    Agents Actions Suppl. 1982;11:25-38 PMID: 6960652
  52. Local effects of synthetic leukotrienes (LTC4, LTD4, LTE4, and LTB4) in human skin.
    J Invest Dermatol. 1983 Feb;80(2):115-9 PMID: 6296237
  53. Isolation and culture of microvascular endothelium from human adipose tissue.
    J Clin Invest. 1983 Jun;71(6):1822-9 PMID: 6306056
  54. Bone-marrow transplantation for inborn error of phagocytic cells associated with defective adherence, chemotaxis, and oxidative response during opsonised particle phagocytosis.
    Lancet. 1983 Aug 27;2(8348):473-6 PMID: 6136643
  55. A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
    J Exp Med. 1983 Dec 1;158(6):1785-1803 PMID: 6196430
  56. Paf-acether (platelet-activating factor) increases microvascular permeability and affects endothelium-granulocyte interaction in microvascular beds.
    Acta Physiol Scand. 1983 Nov;119(3):305-8 PMID: 6659994
  57. Leukotriene B4 action on endothelium mediates augmented neutrophil/endothelial adhesion.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2191-3 PMID: 6326110
  58. Abnormalities of polymorphonuclear leukocyte function associated with a heritable deficiency of high molecular weight surface glycoproteins (GP138): common relationship to diminished cell adherence.
    J Clin Invest. 1984 Aug;74(2):536-51 PMID: 6746906
  59. Subcellular localization of the large subunit of Mo1 (Mo1 alpha; formerly gp 110), a surface glycoprotein associated with neutrophil adhesion.
    J Clin Invest. 1984 Oct;74(4):1280-90 PMID: 6480827
  60. New biological activity following intravascular activation of the complement cascade.
    Nature. 1974 Jun 28;249(460):841-3 PMID: 4834789
  61. Adherence of human polymorphonuclear leukocytes to endothelial monolayers: effects of temperature, divalent cations, and chemotactic factors on the strength of adherence measured with a new centrifugation assay.
    Blood. 1985 Feb;65(2):473-9 PMID: 3967088
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113928
Published
1989-02-00
Pages
637-46
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303725
Subset
IM
Grants
NHLBI NIH HHS · HL-28510 · United States
NHLBI NIH HHS · HL-34063 · United States
NHLBI NIH HHS · HL-36577 · United States
External Links
PubMed source DOI: 10.1172/JCI113928 PMC Full Text View journal details
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