A comparison of snRNP-associated Sm-autoantigens: human N, rat N and human B/B'.

Nucleic acids research ·Vol. 17 ·No. 4 ·1989-02-25 ·Pages 1733-43

Schmauss C, McAllister G, Ohosone Y, Hardin JA, Lerner MR

Abstract

N is a tissue-specific, Sm-epitope bearing, snRNP-associated protein found predominantly in brain. The cDNA sequence encoding human N is compared to those for rat N and human B/B'. The amino acid sequences of human and rat N are 100% conserved. Although the amino acid sequences of N and B/B' are very similar to each other, B/B' contains 50 amino acids which are not present in N. On Northern blots the cDNAs encoding N and B/B' recognize two different RNA species. A comparison of the codon usage, as specified by the open reading frames of N and B/B' as well as results from Southern blots, show that N and B/B' are derived from different genes.

MeSH Terms

Amino Acid Sequence Animals Autoantigens/genetics Base Sequence Blotting, Northern Blotting, Southern Cerebellum/metabolism DNA/genetics Genes Humans Molecular Sequence Data RNA, Messenger/genetics Rats Restriction Mapping Ribonucleoproteins/genetics,immunology Ribonucleoproteins, Small Nuclear

Chemicals

Autoantigens RNA, Messenger Ribonucleoproteins Ribonucleoproteins, Small Nuclear DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Schmauss C

Section of Molecular Neurobiology, Howard Hughes Medical Institute Research Laboratories, New Haven, CT.

McAllister G

Ohosone Y

Hardin J A

Lerner M R

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15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/17.4.1733

Published

1989-02-25

Pages

1733-43

Language

English

Region

England

NLM ID

0411011

PMCID

PMC331831

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1093/nar/17.4.1733 PMC Full Text View journal details

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A comparison of snRNP-associated Sm-autoantigens: human N, rat N and human B/B'.

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