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PMID: 2523380 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic analysis of bacteriophage lambda cIII gene: mRNA structural requirements for translation initiation.

Journal of bacteriology ·Vol. 171 ·No. 5 ·1989-05-00 ·Pages 2563-72

Kornitzer D, Teff D, Altuvia S, Oppenheim AB

Abstract

The bacteriophage lambda cIII gene product regulates the lysogenic pathway. The cIII gene is located in the leftward operon, which is transcribed from the pL promoter. We have previously shown (S. Altuvia and A. B. Oppenheim, J. Bacteriol. 167:415-419, 1986) that mutations that show elevated expression lie within the cIII coding sequence. We isolated mutants that show decreased CIII activity. All the mutations were found to cause a drastic reduction in the rate of initiation of cIII translation. Several mutations were found to be scattered within the first 40 nucleotides of the cIII coding region. Additional mutations affected the AUG initiation codon, the Shine-Dalgarno sequence, and the upstream RNaseIII processing site. Computer folding of the cIII mRNA suggested the presence of two alternative RNA structures. All the mutations within the coding region that reduce expression reduce the stability of one specific mRNA structure (structure B). Mutations that increase expression lie in the loops of this structure and may in fact stabilize it by interfering with the formation of the alternative structure (structure A). Thus, it appears that a specific mRNA secondary structure at the beginning of the cIII coding region is essential for efficient translation, suggesting that changes in mRNA structure regulate cIII expression.

MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Gene Expression Regulation Genes, Viral Hydrogen Bonding Molecular Sequence Data Mutation Nucleic Acid Conformation Peptide Chain Initiation, Translational Ribosomes/metabolism Structure-Activity Relationship Transcription Factors/genetics,metabolism Viral Proteins
Chemicals
Transcription Factors Viral Proteins cIII protein, Bacteriophage lambda
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kornitzer D
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Teff D
Altuvia S
Oppenheim A B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.5.2563-2572.1989
Published
1989-05-00
Pages
2563-72
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209935
Subset
IM
Grants
NIGMS NIH HHS · GM 38694 · United States
External Links
PubMed source DOI: 10.1128/jb.171.5.2563-2572.1989 PMC Full Text View journal details
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