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PMID: 25268621 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

A proteomic analysis of human follicular fluid: comparison between younger and older women with normal FSH levels.

International journal of molecular sciences ·Vol. 15 ·No. 10 ·2014-09-29 ·Pages 17518-40

Hashemitabar M, Bahmanzadeh M, Mostafaie A, Orazizadeh M, Farimani M, Nikbakht R

Abstract

The follicular fluid (FF) is produced during folliculogenesis and contains a variety of proteins that play important roles in follicle development and oocyte maturation. Age-related infertility is usually considered as a problem that can be solved by assisted reproduction technology. Therefore, the identification of novel biomarkers that are linked to reproductive aging is the subject of this study. FF was obtained from healthy younger (20-32 years old) and older (38-42 years old) women undergoing intracytoplasmic sperm injection (ICSI) due to male factor infertility. The FF was analyzed by two-dimensional gel electrophoresis (2-DE). The power of two-dimensional gel electrophoresis and the identification of proteins were exploited using matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry. Twenty three protein spots showed reproducible and significant changes in the aged compared to the young group. Of these, 19 protein spots could be identified using MALDI-TOF-TOF-MS. As a result of MASCOT search, five unique downregulated proteins were identified in the older group. These were identified as serotransferrin, hemopexin precursor, complement C3, C4 and kininogen. A number of protein markers were found that may help develop diagnostic methods of infertility.

MeSH Terms
Adult Age Factors Amino Acid Sequence Body Mass Index Electrophoresis, Gel, Two-Dimensional Female Follicle Stimulating Hormone/blood Follicular Fluid/metabolism Humans Metaphase Molecular Sequence Data Oocytes/cytology,metabolism Ovary/embryology Proteome/analysis Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Transferrin/analysis
Chemicals
Proteome Transferrin Follicle Stimulating Hormone
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hashemitabar Mahmoud
Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1579461357, Iran. [email protected].
Bahmanzadeh Maryam
Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1579461357, Iran. [email protected].
Mostafaie Ali
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah 6714415185, Iran. [email protected].
Orazizadeh Mahmoud
Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1579461357, Iran. [email protected].
Farimani Marzieh
Endometr and Endometriosis Research Center, Hamedan University of Medical Sciences, Hamedan 6517789971, Iran. [email protected].
Nikbakht Roshan
Fertility and Infertility & Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 6193673166, Iran. [email protected].
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Article Info
Journal
International journal of molecular sciences
Abbr.
Int J Mol Sci
ISSN
1422-0067
DOI
10.3390/ijms151017518
Email
[email protected]
[email protected]
[email protected]
[email protected]
[email protected]
[email protected]
Published
2014-09-29
Epub
2014-00-29
Pages
17518-40
Language
English
Region
Switzerland
NLM ID
101092791
PMCID
PMC4227176
Subset
IM
External Links
PubMed source DOI: 10.3390/ijms151017518 PMC Full Text View journal details
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