Home LiteratureArticle Details

PMID: 2531466 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.

Science (New York, N.Y.) ·Vol. 246 ·No. 4935 ·1989-12-08 ·Pages 1275-81

Huse WD, Sastry L, Iverson SA, Kang AS, Alting-Mees M, Burton DR, Benkovic SJ, Lerner RA

Abstract

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

MeSH Terms

Amino Acid Sequence Animals Antibodies, Monoclonal/biosynthesis,genetics Antibody Specificity Antigen-Antibody Reactions Bacteriophage lambda/genetics Base Sequence Cloning, Molecular/methods Escherichia coli/genetics Gene Amplification Gene Library Genetic Vectors Hemocyanins/analogs & derivatives,immunology Immunoglobulin Fab Fragments/biosynthesis Immunoglobulin Fragments/biosynthesis,genetics Mice Molecular Sequence Data Organophosphorus Compounds/immunology Recombinant Proteins/biosynthesis,genetics

Chemicals

Antibodies, Monoclonal Immunoglobulin Fab Fragments Immunoglobulin Fragments Organophosphorus Compounds Recombinant Proteins keyhole limpet hemocyanin phosphonamidate conjugate Hemocyanins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Huse W D

Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.

Sastry L

Iverson S A

Kang A S

Alting-Mees M

Burton D R

Benkovic S J

Lerner R A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.2531466

Published

1989-12-08

Pages

1275-81

Language

English

Region

United States

NLM ID

0404511

Subset

Corrections

CommentIn

PMID: 2588004

External Links

PubMed source DOI: 10.1126/science.2531466 View journal details

Analysis Services

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.

Contact

WeChat Official Account