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PMID: 25315394 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

A comprehensive proteomic approach to identifying capacitation related proteins in boar spermatozoa.

BMC genomics ·Vol. 15 ·2014-10-14 ·Pages 897

Kwon WS, Rahman MS, Lee JS, Kim J, Yoon SJ, Park YJ, You YA, Hwang S, Pang MG

Abstract

Mammalian spermatozoa must undergo capacitation, before becoming competent for fertilization. Despite its importance, the fundamental molecular mechanisms of capacitation are poorly understood. Therefore, in this study, we applied a proteomic approach for identifying capacitation-related proteins in boar spermatozoa in order to elucidate the events more precisely. 2-DE gels were generated from spermatozoa samples in before- and after-capacitation. To validate the 2-DE results, Western blotting and immunocytochemistry were performed with 2 commercially available antibodies. Additionally, the protein-related signaling pathways among identified proteins were detected using Pathway Studio 9.0. We identified Ras-related protein Rab-2, Phospholipid hydroperoxide glutathione peroxidase (PHGPx) and Mitochondrial pyruvate dehydrogenase E1 component subunit beta (PDHB) that were enriched before-capacitation, and NADH dehydrogenase 1 beta subcomplex 6, Mitochondrial peroxiredoxin-5, (PRDX5), Apolipoprotein A-I (APOA1), Mitochondrial Succinyl-CoA ligase [ADP-forming] subunit beta (SUCLA2), Acrosin-binding protein, Ropporin-1A, and Spermadhesin AWN that were enriched after-capacitation (>3-fold) by 2-DE and ESI-MS/MS. SUCLA2 and PDHB are involved in the tricarboxylic acid cycle, whereas PHGPx and PRDX5 are involved in glutathione metabolism. SUCLA2, APOA1 and PDHB mediate adipocytokine signaling and insulin action. The differentially expressed proteins following capacitation are putatively related to sperm functions, such as ROS and energy metabolism, motility, hyperactivation, the acrosome reaction, and sperm-egg interaction. The results from this study elucidate the proteins involved in capacitation, which may aid in the design of biomarkers that can be used to predict boar sperm quality.

MeSH Terms
Animals Gene Expression Regulation Male Proteomics/methods Signal Transduction Sperm Capacitation Spermatozoa/physiology Sus scrofa
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kwon Woo-Sung
Rahman Md Saidur
Lee June-Sub
Kim Jin
Yoon Sung-Jae
Park Yoo-Jin
You Young-Ah
Hwang Seongsoo
Pang Myung-Geol
Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea. [email protected].
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Article Info
Journal
BMC genomics
Abbr.
BMC Genomics
ISSN
1471-2164
DOI
10.1186/1471-2164-15-897
Email
[email protected]
Published
2014-10-14
Epub
2014-00-14
Pages
897
Language
English
Region
England
NLM ID
100965258
PMCID
PMC4287242
Subset
IM
External Links
PubMed source DOI: 10.1186/1471-2164-15-897 PMC Full Text View journal details
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