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PMID: 2535509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Endoplasmic reticulum targeting and glycosylation of hybrid proteins in transgenic tobacco.

The Plant cell ·Vol. 1 ·No. 3 ·1989-03-00 ·Pages 381-90

Iturriaga G, Jefferson RA, Bevan MW

Abstract

The correct compartmentation of proteins to the endomembrane system, mitochondria, or chloroplasts requires an amino-terminal signal peptide. The major tuber protein of potato, patatin, has a signal peptide in common with many other plant storage proteins. When the putative signal peptide of patatin was fused to the bacterial reporter protein beta-glucuronidase, the fusion proteins were translocated to the endoplasmic reticulum in planta and in vitro. In addition, translocated beta-glucuronidase was modified by glycosylation, and the signal peptide was correctly processed. In the presence of an inhibitor of glycosylation, tunicamycin, the enzymatically active form of beta-glucuronidase was assembled in the endoplasmic reticulum. This is the first report of targeting a cytoplasmic protein to the endoplasmic reticulum of plants using a signal peptide.

MeSH Terms
Amino Acid Sequence Carboxylic Ester Hydrolases Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum/metabolism Glucuronidase/genetics,metabolism Glycosylation Molecular Sequence Data Plant Proteins/metabolism Plants, Genetically Modified Plants, Toxic Protein Processing, Post-Translational Protein Sorting Signals/metabolism Recombinant Fusion Proteins/metabolism Tobacco/metabolism Tunicamycin/pharmacology
Chemicals
Plant Proteins Protein Sorting Signals Recombinant Fusion Proteins patatin protein, Solanum tuberosum Tunicamycin Carboxylic Ester Hydrolases Glucuronidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Iturriaga G
Institute of Plant Science Research, Cambridge Laboratory, United Kingdom.
Jefferson R A
Bevan M W
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.1.3.381
Published
1989-03-00
Pages
381-90
Language
English
Region
England
NLM ID
9208688
PMCID
PMC159770
Subset
IM
Grants
NIGMS NIH HHS · GM1078902 · United States
External Links
PubMed source DOI: 10.1105/tpc.1.3.381 PMC Full Text View journal details
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