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PMID: 2535738 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Intracellular localization and DNA-binding properties of human papillomavirus type 18 E6 protein expressed with a baculovirus vector.

Journal of virology ·Vol. 63 ·No. 1 ·1989-01-00 ·Pages 366-74

Grossman SR, Mora R, Laimins LA

Abstract

The E6 protein of human papillomavirus type 18 (HPV-18) is a putative zinc-finger protein that is expressed in HPV-18-induced genital neoplasias. We have studied the biochemical properties of E6 protein synthesized in large amounts with a baculovirus expression vector. When E6 protein was synthesized in insect cells infected with an E6-expressing baculovirus, the protein was localized to both nuclear and membrane fractions, with half-lives of 4 and 2 h, respectively. Changing the first five amino acids of E6 did not alter the pattern of cellular localization of the protein but dramatically increased the half-life of the nuclear component to longer than 30 h and increased the half-life of the membrane component to 8 h. Although the baculovirus-expressed E6 protein bound to double-stranded DNA with high affinity, no sequence specificity for HPV-18 DNA was detected.

MeSH Terms
Animals Blotting, Western Cell Fractionation Cell Line DNA/metabolism DNA-Binding Proteins/analysis,biosynthesis,metabolism Fluorescent Antibody Technique Gene Expression Regulation Genetic Vectors Half-Life Humans Insect Viruses/genetics Metalloproteins/analysis,biosynthesis,metabolism Moths Papillomaviridae/genetics Recombinant Proteins/analysis,biosynthesis,metabolism Zinc
Chemicals
DNA-Binding Proteins Metalloproteins Recombinant Proteins DNA Zinc
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grossman S R
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Mora R
Laimins L A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.1.366-374.1989
Published
1989-01-00
Pages
366-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247692
Subset
IM
Grants
NICHD NIH HHS · 2T32HD-07009-09 · United States
NIGMS NIH HHS · 5T32 GM07821 · United States
NCI NIH HHS · CA 14599 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.1.366-374.1989 PMC Full Text View journal details
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