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PMID: 2536666 Published · ppublish English Journal Article

Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene.

Journal of bacteriology ·Vol. 171 ·No. 1 ·1989-01-00 ·Pages 577-80

Matsuyama A, Yamamoto H, Nakano E

Abstract

The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome.

MeSH Terms
Acetate Kinase/biosynthesis,genetics Amino Acid Sequence Base Sequence Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular Codon/genetics Escherichia coli/genetics,metabolism Genes Genes, Bacterial Molecular Sequence Data Phosphotransferases/genetics Plasmids
Chemicals
Codon Phosphotransferases Acetate Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matsuyama A
Research and Development Division, Kikkoman Corporation, Chiba-ken, Japan.
Yamamoto H
Nakano E
References (21)
21 references, click to expand
  1. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.
    J Mol Biol. 1968 Jul 14;34(2):331-46 PMID: 4938550
  2. Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants.
    J Bacteriol. 1977 Apr;130(1):4-10 PMID: 323237
  3. Energy conservation in chemotrophic anaerobic bacteria.
    Bacteriol Rev. 1977 Mar;41(1):100-80 PMID: 860983
  4. The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli.
    J Gen Microbiol. 1977 Oct;102(2):327-36 PMID: 21941
  5. Purification and properties of acetate kinase from Bacillus stearothermophilus.
    J Biochem. 1978 Jul;84(1):193-203 PMID: 29037
  6. Anaerobic growth of Escherichia coli K12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoroacetate-resistant mutants.
    J Gen Microbiol. 1979 Dec;115(2):259-71 PMID: 393800
  7. In vitro packaging of lambda and cosmid DNA.
    Methods Enzymol. 1979;68:299-309 PMID: 161604
  8. Galactose fermentation by Streptococcus lactis and Streptococcus cremoris: pathways, products, and regulation.
    J Bacteriol. 1980 Nov;144(2):672-82 PMID: 6776093
  9. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  10. Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase.
    J Biol Chem. 1982 Apr 10;257(7):3525-31 PMID: 6277938
  11. Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis.
    J Biol Chem. 1982 Apr 10;257(7):3532-6 PMID: 7037784
  12. Specific mistranslation in hisT mutants of Escherichia coli.
    Mol Gen Genet. 1982;187(3):405-9 PMID: 6757659
  13. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  14. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  15. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  16. Structural features of the hisT operon of Escherichia coli K-12.
    Nucleic Acids Res. 1985 Jul 25;13(14):5297-315 PMID: 2991861
  17. Isolation and characterization of homogeneous acetate kinase from Salmonella typhimurium and Escherichia coli.
    J Biol Chem. 1986 Oct 15;261(29):13487-97 PMID: 3020034
  18. The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons.
    J Biol Chem. 1987 Sep 5;262(25):12209-17 PMID: 3040734
  19. Primary structure of the Escherichia coli folC gene and its folylpolyglutamate synthetase-dihydrofolate synthetase product and regulation of expression by an upstream gene.
    J Biol Chem. 1987 Sep 5;262(25):12337-43 PMID: 3040739
  20. Enzymic formation of adenosine triphosphate with acetyl phosphate as donor in a yeast extract.
    Biochim Biophys Acta. 1963 Jan 8;67:168-70 PMID: 14035672
  21. Fatty acid synthesis by enzyme preparations of Clostridium kluyveri. VI. Reactions of acyl phosphates.
    J Biol Chem. 1950 Jun;184(2):769-93 PMID: 15428461
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.1.577-580.1989
Published
1989-01-00
Pages
577-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209626
Subset
IM
Databases
GENBANK
M22956
External Links
PubMed source DOI: 10.1128/jb.171.1.577-580.1989 PMC Full Text View journal details
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