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PMID: 2537321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of urokinase receptors in monocytelike U937 cells by phorbol ester phorbol myristate acetate.

The Journal of cell biology ·Vol. 108 ·No. 2 ·1989-02-00 ·Pages 693-702

Picone R, Kajtaniak EL, Nielsen LS, Behrendt N, Mastronicola MR, Cubellis MV, Stoppelli MP, Pedersen S, Danø K, Blasi F

Abstract

A specific surface receptor for urokinase plasminogen activator (uPA) recognizes the amino-terminal growth factor-like sequence of uPA, a region independent from and not required for the catalytic activity of this enzyme. The properties of the uPA receptor (uPAR) and the localization and distribution of uPA in tumor cells and tissues suggest that the uPA/uPAR interaction may be important in regulating extracellular proteolysis-dependent processes (e.g., invasion, tissue destruction). Phorbol myristate acetate (PMA), an inducer of U937 cell differentiation to macrophage-like cells, elicits a time- and concentration-dependent increase in the number of uPAR molecules as shown by binding, cross-linking, and immunoprecipitation studies. The effect of PMA is blocked by cycloheximide. Overall, the data indicate that PMA increases the synthesis of uPA. PMA treatment also causes a decrease in the affinity of the uPAR for uPA, thus uncovering another way of regulating the interaction between uPA and uPAR. In addition, the PMA treatment causes a modification of migration of the cross-linked receptor in mono- and bidimensional gel electrophoresis.

MeSH Terms
Cell Count Cell Line Cross-Linking Reagents Electrophoresis, Polyacrylamide Gel Enzyme Precursors Humans Immunosorbent Techniques Molecular Weight Monocytes/metabolism Peptide Fragments/metabolism Plasminogen Activators/metabolism Receptors, Cell Surface/biosynthesis,drug effects,metabolism Receptors, Urokinase Plasminogen Activator Tetradecanoylphorbol Acetate/pharmacology Urokinase-Type Plasminogen Activator/metabolism
Chemicals
Cross-Linking Reagents Enzyme Precursors PLAUR protein, human Peptide Fragments Receptors, Cell Surface Receptors, Urokinase Plasminogen Activator Plasminogen Activators Urokinase-Type Plasminogen Activator Tetradecanoylphorbol Acetate
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Picone R
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Kajtaniak E L
Nielsen L S
Behrendt N
Mastronicola M R
Cubellis M V
Stoppelli M P
Pedersen S
Danø K
Blasi F
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.2.693
Published
1989-02-00
Pages
693-702
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115427
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.108.2.693 PMC Full Text View journal details
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