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PMID: 2542017 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ubiquitous and lymphocyte-specific factors are involved in the induction of the mouse interleukin 2 gene in T lymphocytes.

The EMBO journal ·Vol. 8 ·No. 2 ·1989-02-00 ·Pages 465-73

Serfling E, Barthelmäs R, Pfeuffer I, Schenk B, Zarius S, Swoboda R, Mercurio F, Karin M

Abstract

The immediate upstream region of the mouse interleukin 2 (Il-2) gene harbors a strong transcriptional enhancer. This enhancer contains most, if not all of the sequence elements necessary for the T cell specific induction of the Il-2 gene by the phorbol ester TPA and the plant lectin Concanavalin A. DNase I footprinting studies with fractionated extracts obtained from induced and uninduced E14 T cells revealed numerous recognition sites for potential trans-acting factors. Five of these sites are also recognized by the TPA-activated HeLa cell factors AP-1 and AP-3. Other sites including two TATA-boxes, two purine-rich sequence motifs and two copies of the GGGPuTTTCAA motif are recognized by lymphocyte specific factors. The latter motif is highly conserved between several lymphokine genes and is therefore designated as a T cell element (TCE). In E14 T cells, pentamers of the distal TCEd confer an activity similar to that of the entire Il-2 enhancer, whereas in B and HeLa cells, the TCEd-pentamer is inactive as is the Il-2 enhancer. These data indicate the involvement of the TCEd and its recognition factor(s) in the cell type specific induction of the Il-2 gene during T cell activation.

MeSH Terms
Animals Base Sequence Binding Sites DNA/genetics Deoxyribonuclease I Enhancer Elements, Genetic Gene Products, tat Interleukin-2/biosynthesis,genetics Mice Molecular Sequence Data T-Lymphocytes/immunology Transcription Factors/genetics,metabolism
Chemicals
Gene Products, tat Interleukin-2 Transcription Factors DNA Deoxyribonuclease I
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Serfling E
Institut für Virologie und Immunbiologie, Universität Würzburg, FRG.
Barthelmäs R
Pfeuffer I
Schenk B
Zarius S
Swoboda R
Mercurio F
Karin M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-02-00
Pages
465-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400828
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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