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PMID: 2542621 Published · ppublish English Journal Article

Genetic assignment of multiple E2 gene products in bovine papillomavirus-transformed cells.

Journal of virology ·Vol. 63 ·No. 7 ·1989-07-00 ·Pages 3151-4

Lambert PF, Hubbert NL, Howley PM, Schiller JT

Abstract

The E2 open reading frame of bovine papillomavirus type 1 has been shown genetically to encode at least three transcriptional regulatory factors, and three E2 specific proteins have been recently identified in virally transformed rodent cells. In this study, the genes encoding these E2 specific proteins have been determined. The 48-kilodalton (kDa) protein was identified as the product of a full-length E2 open reading frame cDNA, which confirmed that this polypeptide is the E2 transactivator. The 31-kDa E2 protein species, which is the most abundant E2 specific polypeptide, was identified by analysis of both bovine papillomavirus type 1 mutants and cDNAs to be the previously identified E2 transcriptional repressor, E2-TR, which results from translation initiation at an internal E2 ATG codon. The smallest E2 protein species, the 28-kDa polypeptide, was identified as the product of the E8/E2 fusion gene which results from translation of a spliced mRNA species.

MeSH Terms
Animals Bovine papillomavirus 1/genetics Cell Transformation, Viral DNA, Viral/genetics Gene Products, tat Genes Genes, Viral Oncogene Proteins, Viral/genetics Papillomaviridae/genetics Promoter Regions, Genetic Restriction Mapping Transcription Factors/genetics
Chemicals
DNA, Viral Gene Products, tat Oncogene Proteins, Viral Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lambert P F
Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.
Hubbert N L
Howley P M
Schiller J T
References (29)
29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.7.3151-3154.1989
Published
1989-07-00
Pages
3151-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250873
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.7.3151-3154.1989 PMC Full Text View journal details
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