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PMID: 2542764 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of 2',5'-oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor.

Molecular and cellular biology ·Vol. 9 ·No. 3 ·1989-03-00 ·Pages 1060-8

Garcia-Blanco MA, Lengyel P, Morrison E, Brownlee C, Stiles CD, Rutherford M, Hannigan G, Williams BR

Abstract

In murine BALB/c 3T3 cell cultures, either beta interferon or platelet-derived growth factor (PDGF) enhanced expression of the 2',5'-oligoadenylate synthetase mRNA and protein. The time course of induction in response to beta interferon was similar to that in response to PDGF. Of several growth factors known to be present in clotted blood serum (i.e., epidermal growth factor, transforming growth factor beta, and PDGF), only PDGF enhanced expression of 2',5'-oligoadenylate synthetase. The linkage of an interferon response element-containing segment from the 5'-flanking region of a human or murine 2',-5'-oligoadenylate synthetase gene made a heterologous gene responsive to interferon. The expression of such a gene construct in transfected cells was also induced by PDGF. Induction by PDGF was inhibited by mono- or polyclonal antibodies to murine interferon, which suggested that induction by PDGF requires interferon. Both PDGF and interferon induced nuclear factors that bound to this interferon response element-containing segment in vitro.

MeSH Terms
2',5'-Oligoadenylate Synthetase/biosynthesis,genetics Animals Clone Cells/drug effects,metabolism Gene Expression Regulation/drug effects Gene Products, tat Interferon Type I/pharmacology Platelet-Derived Growth Factor/pharmacology RNA, Messenger/biosynthesis,genetics Transcription Factors/metabolism
Chemicals
Gene Products, tat Interferon Type I Platelet-Derived Growth Factor RNA, Messenger Transcription Factors 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Garcia-Blanco M A
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.
Lengyel P
Morrison E
Brownlee C
Stiles C D
Rutherford M
Hannigan G
Williams B R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.3.1060-1068.1989
Published
1989-03-00
Pages
1060-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362696
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.3.1060-1068.1989 PMC Full Text View journal details
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