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PMID: 2543131 Published · ppublish English Journal Article

A syncytia assay for human immunodeficiency virus type I (HIV-I) envelope protein and its use in studying HIV-I mutations.

Virology ·Vol. 170 ·No. 2 ·1989-06-00 ·Pages 566-70

Felser JM, Klimkait T, Silver J

Abstract

We describe a syncytia assay that utilizes a noninfectious plasmid (pEVd1443) derived from human immunodeficiency virus type I (HIV-I). This plasmid carries a large deletion of gag-pol sequences but expresses HIV-I envelope proteins and induces syncytia following transfection into HeLa-CD4 cells. This plasmid was used to study the effects of mutations in the gp 120 and gp41 portions of the envelope gene of HIV-I.

MeSH Terms
Antigens, Differentiation, T-Lymphocyte/genetics Blotting, Western Gene Expression Regulation Gene Products, gag Gene Products, tat HIV Antigens/genetics HIV Envelope Protein gp120 HIV Envelope Protein gp160 HIV Envelope Protein gp41 HIV-1/genetics,immunology HeLa Cells Humans Mutation Plasmids Precipitin Tests Retroviridae Proteins/analysis,genetics Transcription Factors/genetics Transfection Viral Envelope Proteins/analysis,genetics tat Gene Products, Human Immunodeficiency Virus
Chemicals
Antigens, Differentiation, T-Lymphocyte Gene Products, gag Gene Products, tat HIV Antigens HIV Envelope Protein gp120 HIV Envelope Protein gp160 HIV Envelope Protein gp41 Retroviridae Proteins Transcription Factors Viral Envelope Proteins tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Felser J M
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Klimkait T
Silver J
Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
Published
1989-06-00
Pages
566-70
Language
English
Region
United States
NLM ID
0110674
Subset
IM
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