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PMID: 2544029 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA looping generated by DNA bending protein IHF and the two domains of lambda integrase.

Science (New York, N.Y.) ·Vol. 244 ·No. 4911 ·1989-06-23 ·Pages 1457-61

Moitoso de Vargas L, Kim S, Landy A

Abstract

The multiprotein-DNA complexes that participate in bacteriophage lambda site-specific recombination were used to study the combined effect of protein-induced bending and protein-mediated looping of DNA. The protein integrase (Int) is a monomer with two autonomous DNA binding domains of different sequence specificity. Stimulation of Int binding and cleavage at the low affinity core-type DNA sites required interactions with the high affinity arm-type sites and depended on simultaneous binding of the sequence-specific DNA bending protein IHF (integration host factor). The bivalent DNA binding protein is positioned at high affinity sites and directed, by a DNA bending protein, to interactions with distant lower affinity sites. Assembly of this complex is independent of protein-protein interactions.

MeSH Terms
Bacterial Proteins/metabolism,pharmacology Bacteriophage lambda/enzymology,genetics Binding Sites DNA Nucleotidyltransferases/metabolism DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/genetics,metabolism DNA, Viral/genetics,metabolism DNA-Binding Proteins/metabolism Integrases Integration Host Factors Mutation Nucleic Acid Conformation/drug effects Recombination, Genetic
Chemicals
Bacterial Proteins DNA Transposable Elements DNA, Bacterial DNA, Viral DNA-Binding Proteins Integration Host Factors DNA Nucleotidyltransferases Integrases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moitoso de Vargas L
Division of Biology and Medicine, Brown University, Providence, RI 02912.
Kim S
Landy A
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48 references, click to expand
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.2544029
Published
1989-06-23
Pages
1457-61
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC1892171
Subset
IM
Grants
NIGMS NIH HHS · R01 GM033928 · United States
NIGMS NIH HHS · R01 GM062723 · United States
NIAID NIH HHS · AI-13544 · United States
NIGMS NIH HHS · GM-33928 · United States
External Links
PubMed source DOI: 10.1126/science.2544029 PMC Full Text View journal details
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