Home LiteratureArticle Details
PMID: 2544630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CD3-negative lymphoproliferative disease of granular lymphocytes containing Epstein-Barr viral DNA.

The Journal of clinical investigation ·Vol. 84 ·No. 1 ·1989-07-00 ·Pages 51-5

Kawa-Ha K, Ishihara S, Ninomiya T, Yumura-Yagi K, Hara J, Murayama F, Tawa A, Hirai K

Abstract

Lymphoproliferative disease of granular lymphocytes (LDGL) is a heterogeneous disorder and the pathogenesis is likely to be complex. Some patients with chronic active EBV (CAEBV) infection also have LDGL. To investigate the relationship between EBV infection and the pathogenesis of LDGL, we conducted a survey for EBV DNA sequences by Southern blot analysis of DNA obtained from the peripheral blood of seven patients with LDGL, including one with CAEBV infection. Interestingly, EBV DNA was detected in the sample from the patient with CAEBV infection, and in the samples from four other patients with CD3-LDGL. Moreover, a single band for the joined termini of the EBV genome was demonstrated in two samples, suggesting a clonal disorder of those LDGL. These findings strongly suggest that EBV may play a pathogenic role in some cases of LDGL.

MeSH Terms
Adolescent Adult Aged Antibodies, Viral/immunology Antigens, Differentiation, T-Lymphocyte/immunology Antigens, Viral/immunology Blotting, Southern CD3 Complex Capsid/immunology Child Chronic Disease DNA, Viral/analysis,genetics Female Granulocytes/immunology,microbiology Herpesvirus 4, Human/genetics,immunology Humans Lymphoproliferative Disorders/immunology,microbiology Male Middle Aged Nucleic Acid Hybridization Receptors, Antigen, T-Cell/genetics,immunology
Chemicals
Antibodies, Viral Antigens, Differentiation, T-Lymphocyte Antigens, Viral CD3 Complex DNA, Viral Receptors, Antigen, T-Cell
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kawa-Ha K
Department of Pediatrics, Osaka University Hospital, Japan.
Ishihara S
Ninomiya T
Yumura-Yagi K
Hara J
Murayama F
Tawa A
Hirai K
References (25)
25 references, click to expand
  1. Two tandemly organized human genes encoding the T-cell gamma constant-region sequences show multiple rearrangement in different T-cell types.
    Nature. 1985 Aug 1-7;316(6027):464-6 PMID: 2991773
  2. Human natural killer cells: biologic and pathologic aspects.
    Lab Invest. 1984 May;50(5):489-513 PMID: 6201674
  3. Leu 11+ T gamma cell chronic lymphocytic leukemia with partially activated natural killer function and its further activation by recombinant IL2 in vitro.
    Blood. 1986 Oct;68(4):846-52 PMID: 2944554
  4. Heterogeneity of large granular lymphocyte proliferations: delineation of two major subtypes.
    Blood. 1986 Nov;68(5):1142-53 PMID: 3490288
  5. The structure of the termini of the Epstein-Barr virus as a marker of clonal cellular proliferation.
    Cell. 1986 Dec 26;47(6):883-9 PMID: 3022942
  6. Successful treatment of chronic active Epstein-Barr virus infection with recombinant interleukin-2.
    Lancet. 1987 Jan 17;1(8525):154 PMID: 2879982
  7. Concomitant rearrangements of T-cell beta- and gamma-chain genes in childhood T-lineage leukemia/lymphoma.
    Leuk Res. 1987;11(8):739-45 PMID: 3498098
  8. The lymphoproliferative disease of granular lymphocytes. A heterogeneous disorder ranging from indolent to aggressive conditions.
    Cancer. 1987 Dec 15;60(12):2971-8 PMID: 3677021
  9. Remarkable depression of CD4+2H4+ T cells in severe chronic active Epstein-Barr virus infection.
    Scand J Immunol. 1987 Dec;26(6):769-73 PMID: 2827299
  10. B-cell lymphoproliferation and lymphomagenesis are associated with clonotypic intracellular terminal regions of the Epstein-Barr virus.
    J Virol. 1988 Mar;62(3):962-9 PMID: 2828691
  11. T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections.
    N Engl J Med. 1988 Mar 24;318(12):733-41 PMID: 2831453
  12. Structure of the human B lymphocyte receptor for C3d and the Epstein-Barr virus and relatedness to other members of the family of C3/C4 binding proteins.
    J Exp Med. 1988 Mar 1;167(3):1047-66 PMID: 2832506
  13. The expression of the p75 subunit of interleukin 2 receptor in Tac negative leukemic cells of two patients with large granular lymphocytic leukemia.
    Blood. 1988 Apr;71(4):1161-4 PMID: 2833327
  14. Epstein-Barr virus genome-positive T lymphocytes in a boy with chronic active EBV infection associated with Kawasaki-like disease.
    Nature. 1988 Jun 2;333(6172):455-7 PMID: 2836734
  15. Granular lymphocyte proliferative disorders: report of 12 cases and review of the literature.
    Leukemia. 1988 Oct;2(10):617-27 PMID: 3050292
  16. Negative association between the human T-cell leukaemia virus type I and large granular lymphocyte leukaemia in Japan.
    Lancet. 1988 Oct 22;2(8617):962 PMID: 2902406
  17. Clonal T-cell lymphoproliferation containing Epstein-Barr (EB) virus DNA in a patient with chronic active EB virus infection.
    Jpn J Cancer Res. 1989 Feb;80(2):99-101 PMID: 2542204
  18. Epstein-Barr virus specific diagnostic tests in infectious mononucleosis.
    Hum Pathol. 1974 Sep;5(5):551-65 PMID: 4368691
  19. Characterization of a human B cell-specific antigen (B2) distinct from B1.
    J Immunol. 1981 May;126(5):1941-7 PMID: 6163824
  20. Characteristics of human large granular lymphocytes and relationship to natural killer and K cells.
    J Exp Med. 1981 Mar 1;153(3):569-82 PMID: 6166701
  21. Immunologic evaluation of T chronic lymphocyte leukemia cells: correlations among phenotype, functional activities, and morphology.
    Blood. 1982 Apr;59(4):688-95 PMID: 6977387
  22. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  23. Use of cloned probes to detect Epstein-Barr viral DNA in tissues of patients with neoplastic and lymphoproliferative diseases.
    J Infect Dis. 1983 Dec;148(6):967-77 PMID: 6317774
  24. A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains.
    Nature. 1984 Mar 8-14;308(5955):145-9 PMID: 6336315
  25. Chromosomal localization of the Epstein-Barr virus (EBV) genome in Bloom's syndrome B-lymphoblastoid cell lines transformed with EBV.
    Chromosoma. 1985;93(2):157-64 PMID: 3002731
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114168
Published
1989-07-00
Pages
51-5
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303951
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI114168 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2