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PMID: 2544874 Published · ppublish English Comparative Study Journal Article

Structure of simian immunodeficiency virus regulatory genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 13 ·1989-07-00 ·Pages 4813-7

Colombini S, Arya SK, Reitz MS, Jagodzinski L, Beaver B, Wong-Staal F

Abstract

Three full-length cDNA clones were obtained from cells infected with the simian immunodeficiency virus (SIV) isolated from captive macaques (SIVMAC). Nucleotide sequence analyses suggested that these represented mRNA for the SIV MAC genes tat, rev (formerly, art/trs), and nef (formerly, 3'orf). The putative tat-specific clone was active in trans-activation of the SIV MAC long terminal repeat in COS-1 and Jurkat cells. In contrast, the human immunodeficiency virus 1 long terminal repeat was significantly trans-activated only in the COS-1 cells. This suggests that trans-activation by the SIV tat gene is modulated by cell-specific factors. The structure of all of the clones suggested an mRNA splicing pattern more complex than that described for human immunodeficiency virus 1.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA, Viral/genetics Gene Expression Regulation Gene Products, nef Gene Products, rev Gene Products, tat Genes, Regulator Genes, Viral HIV-1/genetics HIV-2/genetics Humans Macaca Molecular Sequence Data Oncogene Proteins, Viral/genetics Plasmids RNA, Messenger/genetics Retroviridae Proteins/genetics Sequence Homology, Nucleic Acid Simian Immunodeficiency Virus/genetics Transcription Factors/genetics Transfection Viral Proteins/genetics nef Gene Products, Human Immunodeficiency Virus rev Gene Products, Human Immunodeficiency Virus tat Gene Products, Human Immunodeficiency Virus
Chemicals
DNA, Viral Gene Products, nef Gene Products, rev Gene Products, tat Oncogene Proteins, Viral RNA, Messenger Retroviridae Proteins Transcription Factors Viral Proteins nef Gene Products, Human Immunodeficiency Virus rev Gene Products, Human Immunodeficiency Virus tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Colombini S
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Arya S K
Reitz M S
Jagodzinski L
Beaver B
Wong-Staal F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.13.4813
Published
1989-07-00
Pages
4813-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297505
Subset
IM
Databases
GENBANK
M23165
External Links
PubMed source DOI: 10.1073/pnas.86.13.4813 PMC Full Text View journal details
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