cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 13 ·1989-07-00 ·Pages 4917-21

Claesson-Welsh L, Eriksson A, Westermark B, Heldin CH

Abstract

The primary structure of the human A-type receptor for platelet-derived growth factor (PDGF) has been determined. A 6.5-kilobase (kb) transcript was identified through low-stringency hybridization with a probe derived from the B-type PDGF receptor cDNA. The sequence of a cDNA clone corresponding to the 6.5-kb transcript contains an open reading frame that predicts a 1089-amino acid growth factor receptor-like molecule, which displays 44% overall amino acid similarity with the PDGF B-type receptor. The two receptors have a similar domain organization, with five immunoglobulin-like domains extracellularly and an intracellular split protein tyrosine kinase domain. Transfection of the new cDNA into COS cells led to the expression of a protein specifically recognized by an antiserum previously shown to react with the PDGF A-type receptor. The expressed protein was shown to display high-affinity binding of all three 125I-labeled dimeric forms of PDGF A and B chains in a manner that is characteristic for the PDGF A-type receptor.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA/genetics,isolation & purification Humans Molecular Sequence Data Nucleic Acid Hybridization Platelet-Derived Growth Factor/metabolism Receptors, Cell Surface/genetics Receptors, Platelet-Derived Growth Factor Sequence Homology, Nucleic Acid Structure-Activity Relationship Transfection

Chemicals

Platelet-Derived Growth Factor Receptors, Cell Surface DNA Receptors, Platelet-Derived Growth Factor

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Claesson-Welsh L

Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.

Eriksson A

Westermark B

Heldin C H

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.13.4917

Published

1989-07-00

Pages

4917-21

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC297526

Subset

Databases

GENBANK

M22734

External Links

PubMed source DOI: 10.1073/pnas.86.13.4917 PMC Full Text View journal details

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cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor.

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