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PMID: 2545628 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of additional virulence determinants on the pYV plasmid of Yersinia enterocolitica W227.

Infection and immunity ·Vol. 57 ·No. 8 ·1989-08-00 ·Pages 2534-41

Mulder B, Michiels T, Simonet M, Sory MP, Cornelis G

Abstract

This paper describes the mutagenesis of the pYV plasmid from Yersinia enterocolitica W22703 (serotype O:9) with Tn2507, a new element generating operon fusions. Analysis of the mutants allowed the identification of an additional Yop protein called Yop20 and the mapping of yop20, yop44, yop48, and lcrV, the gene encoding the V antigen. The last gene appeared to be part of an operon that also may contain yop37 and yop44. At 37 degrees C, mutants affected in this operon grew poorly, irrespective of the presence of Ca2+, or they even died in the presence of Ca2+. This operon is thus involved in the regulation by Ca2+, and we called it car, for Ca2+ regulation. It is presumably the Y. enterocolitica counterpart of the lcrGVH operon of Yersinia pestis. Transcription of yop20 and of the car operon was strongly regulated by temperature and only slightly by calcium. Hence, these genes behaved like the other genes of the yop regulon. Mutants affected in yop20 or in yop48 were markedly less virulent for the desferrioxamine-treated mouse than was the parental strain. Yop20 and Yop48 thus probably are Yersinia virulence factors.

MeSH Terms
Animals Antigens, Bacterial/genetics Bacterial Proteins/genetics DNA Transposable Elements Female Mice Mice, Inbred ICR Mutation Operon Plasmids Restriction Mapping Virulence Yersinia enterocolitica/genetics,pathogenicity
Chemicals
Antigens, Bacterial Bacterial Proteins DNA Transposable Elements
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mulder B
Unité de Microbiologie, Université Catholique de Louvain, Brussels, Belgium.
Michiels T
Simonet M
Sory M P
Cornelis G
References (39)
39 references, click to expand
  1. Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from Escherichia coli.
    J Gen Microbiol. 1975 Apr;87(2):285-91 PMID: 1095683
  2. The basis of virulence in Pasteurella pestis: an antigen determining virulence.
    Br J Exp Pathol. 1956 Oct;37(5):481-93 PMID: 13374206
  3. The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence.
    Gene. 1980 Dec;12(3-4):179-90 PMID: 7019009
  4. Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.
    Gene. 1982 Dec;20(2):305-16 PMID: 6299895
  5. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development.
    Nature. 1983 Dec 8-14;306(5943):551-7 PMID: 6646234
  6. Identification of a transposon Tn3 sequence required for transposition immunity.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6765-9 PMID: 6316324
  7. Genetically manipulated virulence of Yersinia enterocolitica.
    Infect Immun. 1984 Oct;46(1):105-10 PMID: 6480101
  8. A restriction map of virulence plasmid pVYE439-80 from a serogroup 9 Yersinia enterocolitica strain.
    Plasmid. 1984 Jul;12(1):67-70 PMID: 6093172
  9. Genetic analysis of the low calcium response in Yersinia pestis mu d1(Ap lac) insertion mutants.
    J Bacteriol. 1984 Dec;160(3):842-8 PMID: 6094509
  10. Expression of antigens encoded by the virulence plasmid of Yersinia enterocolitica under different growth conditions.
    Infect Immun. 1985 Jan;47(1):183-90 PMID: 3965395
  11. Effects of iron and desferrioxamine on infections with Yersinia enterocolitica.
    Infect Immun. 1985 Mar;47(3):774-9 PMID: 3972453
  12. Expression of the temperature-inducible outer membrane proteins of yersiniae.
    Infect Immun. 1985 Apr;48(1):234-40 PMID: 3980086
  13. Genetic analysis of virulence plasmid from a serogroup 9 Yersinia enterocolitica strain: role of outer membrane protein P1 in resistance to human serum and autoagglutination.
    Infect Immun. 1985 Jun;48(3):782-6 PMID: 2987130
  14. A single genetic locus encoded by Yersinia pseudotuberculosis permits invasion of cultured animal cells by Escherichia coli K-12.
    Nature. 1985 Sep 19-25;317(6034):262-4 PMID: 2995819
  15. Isolation and characterization of Ca2+-blind mutants of Yersinia pestis.
    J Bacteriol. 1985 Nov;164(2):704-11 PMID: 2997127
  16. Virulence genes regulated at the transcriptional level by Ca2+ in Yersinia pestis include structural genes for outer membrane proteins.
    Infect Immun. 1986 Feb;51(2):445-54 PMID: 3002984
  17. Temperature-controlled plasmid regulon associated with low calcium response in Yersinia pestis.
    J Bacteriol. 1986 Feb;165(2):443-7 PMID: 3944056
  18. Tn951 derivatives designed for high-frequency plasmid-specific transposition and deletion mutagenesis.
    Gene. 1986;43(3):175-81 PMID: 3017811
  19. A low-Ca2+ response operon encodes the V antigen of Yersinia pestis.
    Infect Immun. 1986 Nov;54(2):428-34 PMID: 3021629
  20. Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media.
    Infect Immun. 1986 Nov;54(2):561-7 PMID: 3770952
  21. Yersinia enterocolitica, a primary model for bacterial invasiveness.
    Rev Infect Dis. 1987 Jan-Feb;9(1):64-87 PMID: 3547579
  22. Plasmid-mediated surface fibrillae of Yersinia pseudotuberculosis and Yersinia enterocolitica: relationship to the outer membrane protein YOP1 and possible importance for pathogenesis.
    Infect Immun. 1987 Sep;55(9):2247-54 PMID: 2887516
  23. Regulation of expression of V antigen and outer membrane proteins in Yersinia pestis.
    Contrib Microbiol Immunol. 1987;9:173-8 PMID: 3665495
  24. Identification and mapping of the temperature-inducible, plasmid-encoded proteins of Yersinia spp.
    Infect Immun. 1988 Feb;56(2):343-8 PMID: 3338844
  25. Evidence for two genetic loci in Yersinia enterocolitica that can promote invasion of epithelial cells.
    Infect Immun. 1988 May;56(5):1242-8 PMID: 2833444
  26. The virulence protein Yop5 of Yersinia pseudotuberculosis is regulated at transcriptional level by plasmid-plB1-encoded trans-acting elements controlled by temperature and calcium.
    Mol Microbiol. 1988 Jan;2(1):121-33 PMID: 2835586
  27. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR.
    J Bacteriol. 1988 Jun;170(6):2575-83 PMID: 2836362
  28. The plasmid-encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription.
    Mol Microbiol. 1988 Mar;2(2):237-45 PMID: 2837614
  29. Inhibition of phagocytosis in Yersinia pseudotuberculosis: a virulence plasmid-encoded ability involving the Yop2b protein.
    Infect Immun. 1988 Aug;56(8):2139-43 PMID: 3294185
  30. In vitro expression of a 22-kilodalton Yersinia pestis polypeptide immunologically related to the 25-kilodalton plasmid-encoded protein of the three pathogenic Yersinia species.
    Infect Immun. 1988 Oct;56(10):2576-80 PMID: 3047061
  31. Yersinia enterocolitica O:9 as a potential live oral carrier for protective antigens.
    Microb Pathog. 1988 Jun;4(6):431-42 PMID: 3143043
  32. Homology between virF, the transcriptional activator of the Yersinia virulence regulon, and AraC, the Escherichia coli arabinose operon regulator.
    J Bacteriol. 1989 Jan;171(1):254-62 PMID: 2644192
  33. The replication, partition and yop regulation of the pYV plasmids are highly conserved in Yersinia enterocolitica and Y. pseudotuberculosis.
    J Gen Microbiol. 1988 Jun;134(6):1525-34 PMID: 3221196
  34. A plasmid-encoded regulatory gene, rns, required for expression of the CS1 and CS2 adhesins of enterotoxigenic Escherichia coli.
    Proc Natl Acad Sci U S A. 1989 Feb;86(3):963-7 PMID: 2563591
  35. Molecular cloning and expression of calcium-regulated, plasmid-coded proteins of Y. pseudotuberculosis.
    Microb Pathog. 1987 Feb;2(2):123-37 PMID: 3507554
  36. Transcription of the yop regulon from Y. enterocolitica requires trans acting pYV and chromosomal genes.
    Microb Pathog. 1987 May;2(5):367-79 PMID: 3507556
  37. Nucleotide sequence and transcription analysis of yop51 from Yersinia enterocolitica W22703.
    Microb Pathog. 1988 Dec;5(6):449-59 PMID: 3244311
  38. Genetic analysis of the plasmid region controlling virulence in Yersinia enterocolitica 0:9 by Mini-Mu insertions and lac gene fusions.
    Microb Pathog. 1986 Aug;1(4):349-59 PMID: 2854600
  39. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica.
    Infect Immun. 1980 Feb;27(2):682-5 PMID: 7380546
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.8.2534-2541.1989
Published
1989-08-00
Pages
2534-41
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313482
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.8.2534-2541.1989 PMC Full Text View journal details
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