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PMID: 2551673 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overproduction of the regulatory subunit of the cAMP-dependent protein kinase blocks the differentiation of Dictyostelium discoideum.

The EMBO journal ·Vol. 8 ·No. 7 ·1989-07-00 ·Pages 2039-43

Simon MN, Driscoll D, Mutzel R, Part D, Williams J, Véron M

Abstract

During the aggregation of Dictyostelium discoideum extracellular cAMP is known to act as a chemotractant and as an inducer of cellular differentiation. However, its intracellular role as a second messenger remains obscure. We have constructed a fusion gene consisting of the cDNA encoding the regulatory subunit (R) of the cAMP-dependent protein kinase fused to the promoter and N-terminal-proximal sequences of a Dictyostelium actin gene. Stable transformants, containing multiple copies of this gene, overproduce the R subunit which accumulates prematurely relative to the endogenous protein. These transformants fail to aggregate. Detailed analysis has shown that they are blocked at interphase, the period prior to aggregation, and that they are severely defective in most responses to cAMP including the induction of gene expression. Our observations suggest that intracellular cAMP acts, presumably by activation of the catalytic subunit of the cAMP-dependent protein kinase, to facilitate early development.

MeSH Terms
Blotting, Western Carrier Proteins/biosynthesis,genetics Cyclic AMP/metabolism DNA, Fungal/genetics Dictyostelium/enzymology,genetics,growth & development Intracellular Signaling Peptides and Proteins Plasmids Protein Kinases/genetics RNA, Fungal/genetics,isolation & purification
Chemicals
Carrier Proteins DNA, Fungal Intracellular Signaling Peptides and Proteins RNA, Fungal protein kinase modulator Cyclic AMP Protein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Simon M N
Unite de Biochimie Cellulaire, Institut Pasteur, Paris, France.
Driscoll D
Mutzel R
Part D
Williams J
Véron M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-07-00
Pages
2039-43
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401086
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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