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PMID: 2552406 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effect of E. coli host strain on the consensus sequence of regions of the human L1 transposon.

Nucleic acids research ·Vol. 17 ·No. 18 ·1989-09-25 ·Pages 7229-39

Crowther PJ, Cartwright AL, Hocking A, Jefferson S, Ford MD, Woodcock DM

Abstract

We have used highly methylation tolerant host strains to clone hyper- and hypo-methylated genomic elements from different regions of the same family of long interspersed repetitive elements from human DNA, specifically the 1.8 kilobase (kb) and 1.2kb KpnI fragments from members of the L1 family of transposable elements in which respectively some 18% and 2.7% of cytosines are methylated in vivo in human spleen DNA. The consensus of the DNA sequences of the ends of 13 clones from the hypomethylated region of human L1 agreed exactly with the consensus derived previously from clones made using conventional host strains. However the sequences of 18 of our clones from the 5' end of the hypermethylated region differed significantly from the sequences of clones made using conventional hosts (P less than 0.0001). The 5' region of the 1.8kb L1 region is a CpG island which, in human somatic tissue, appears to be maintained in a highly methylated state, including methylation at sites other than CpG dinucleotides. The consensus sequence of this region also has features suggestive of a previously unrecognized open reading frame.

MeSH Terms
Base Sequence Blotting, Southern Cloning, Molecular DNA Transposable Elements Escherichia coli/genetics Genes Genetic Variation Humans Methylation Molecular Sequence Data Repetitive Sequences, Nucleic Acid
Chemicals
DNA Transposable Elements
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Crowther P J
Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia.
Cartwright A L
Hocking A
Jefferson S
Ford M D
Woodcock D M
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26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.18.7229
Published
1989-09-25
Pages
7229-39
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334803
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.18.7229 PMC Full Text View journal details
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