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PMID: 25540389 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer.

Molecular & cellular proteomics : MCP ·Vol. 14 ·No. 3 ·2015-03-00 ·Pages 499-509

Xu J, Li L, Yu G, Ying W, Gao Q, Zhang W, Li X, Ding C, Jiang Y, Wei D, Duan S, Lei Q, Li P, Shi T, Qian X, Qin J, Jia L

Abstract

The neddylation-cullin-RING E3 ligase (CRL) pathway has recently been identified as a potential oncogenic event and attractive anticancer target; however, its underlying mechanisms have not been well elucidated. In this study, RhoB, a well known tumor suppressor, was identified and validated with an iTRAQ-based quantitative proteomic approach as a new target of this pathway in liver cancer cells. Specifically, cullin 2-RBX1 E3 ligase, which requires NEDD8 conjugation for its activation, interacted with RhoB and promoted its ubiquitination and degradation. In human liver cancer tissues, the neddylation-CRL pathway was overactivated and reversely correlated with RhoB levels. Moreover, RhoB accumulation upon inhibition of the neddylation-CRL pathway for anticancer therapy contributed to the induction of tumor suppressors p21 and p27, apoptosis, and growth suppression. Our findings highlight the degradation of RhoB via the neddylation-CRL pathway as an important molecular event that drives liver carcinogenesis and RhoB itself as a pivotal effector for anticancer therapy targeting this oncogenic pathway.

MeSH Terms
Carrier Proteins/metabolism Cell Line, Tumor Cullin Proteins/metabolism HCT116 Cells Hep G2 Cells Human Umbilical Vein Endothelial Cells Humans Liver Neoplasms/metabolism MCF-7 Cells NEDD8 Protein Proteomics/methods Signal Transduction Ubiquitins/metabolism rhoB GTP-Binding Protein/metabolism
Chemicals
CUL2 protein, human Carrier Proteins Cullin Proteins NEDD8 Protein NEDD8 protein, human RBX1 protein, human Ubiquitins rhoB GTP-Binding Protein
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Xu Junfeng
From the ‡Cancer Institute, Fudan University Shanghai Cancer Center, §Department of Oncology and ¶Institutes of Biomedical Sciences, Shanghai Medical College, and.
Li Lihui
From the ‡Cancer Institute, Fudan University Shanghai Cancer Center, §Department of Oncology and.
Yu Guangyang
From the ‡Cancer Institute, Fudan University Shanghai Cancer Center, §Department of Oncology and.
Ying Wantao
‖State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; **National Engineering Research Center for Protein Drugs, Beijing 102206, China;
Gao Qiang
‡‡Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China;
Zhang Wenjuan
From the ‡Cancer Institute, Fudan University Shanghai Cancer Center, §Department of Oncology and.
Li Xianyu
‖State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; **National Engineering Research Center for Protein Drugs, Beijing 102206, China;
Ding Chen
‖State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; **National Engineering Research Center for Protein Drugs, Beijing 102206, China;
Jiang Yanan
From the ‡Cancer Institute, Fudan University Shanghai Cancer Center, §Department of Oncology and.
Wei Dongping
From the ‡Cancer Institute, Fudan University Shanghai Cancer Center, §Department of Oncology and.
Duan Shengzhong
§§Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;
Lei Qunying
¶Institutes of Biomedical Sciences, Shanghai Medical College, and.
Li Peng
¶¶Center for Bioinformatics and Computational Biology and Institute of Biomedical Sciences, School of Life Science, East China Normal University, Shanghai 200241, China.
Shi Tieliu
¶¶Center for Bioinformatics and Computational Biology and Institute of Biomedical Sciences, School of Life Science, East China Normal University, Shanghai 200241, China.
Qian Xiaohong
‖State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; **National Engineering Research Center for Protein Drugs, Beijing 102206, China;
Qin Jun
‖State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; **National Engineering Research Center for Protein Drugs, Beijing 102206, China;
Jia Lijun
From the ‡Cancer Institute, Fudan University Shanghai Cancer Center, §Department of Oncology and [email protected].
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Article Info
Journal
Molecular & cellular proteomics : MCP
Abbr.
Mol Cell Proteomics
ISSN
1535-9484
Published
2015-03-00
Epub
2014-00-24
Pages
499-509
Language
English
Region
United States
NLM ID
101125647
PMCID
PMC4349972
Subset
IM
Analysis Services
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