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PMID: 2557541 Published · ppublish English Journal Article

UV-induced early-domain binding factor as the limiting component of simian virus 40 DNA amplification in rodent cells.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 4812-18

Lücke-Huhle C, Mai S, Herrlich P

Abstract

UV radiation and other carcinogenic agents induce an increase in DNA-binding activity to the early domain of the simian virus 40 (SV40) minimal origin in both SV40-permissive and SV40-nonpermissive cells. The increase is due to posttranslational modification of a preexisting protein, since it occurs in the presence of cycloheximide or anisomycin. Binding of this factor is an absolute requirement for the UV-induced SV40 DNA amplification in Co631 cells in vivo. A synthetic double-stranded oligonucleotide covering the early domain sequence totally blocked the UV-induced amplification in competition experiments. Point mutants of the sequence and unrelated oligonucleotides which could not bind the factor also did not block SV40 amplification. Inhibitors of protein synthesis caused an immediate increase of both early-domain factor activity (perhaps by prolonging mRNA half-life for the factor or for a modifying enzyme) and DNA amplification. The effects of UV and cycloheximide on SV40 amplification were superaddition.

MeSH Terms
Animals Anisomycin/pharmacology Base Sequence Binding, Competitive Cell Line Cricetinae Cycloheximide/pharmacology DNA Damage DNA, Viral/genetics,metabolism DNA-Binding Proteins/metabolism Gene Amplification/drug effects,radiation effects Molecular Sequence Data Mutation Oligodeoxyribonucleotides/metabolism Protein Biosynthesis Simian virus 40/genetics Ultraviolet Rays
Chemicals
DNA, Viral DNA-Binding Proteins Oligodeoxyribonucleotides Anisomycin Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lücke-Huhle C
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, Federal Republic of Germany.
Mai S
Herrlich P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.4812-4818.1989
Published
1989-11-00
Pages
4812-18
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363630
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.11.4812-4818.1989 PMC Full Text View journal details
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