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PMID: 2563996 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae.

Journal of bacteriology ·Vol. 171 ·No. 3 ·1989-03-00 ·Pages 1262-70

Gerlach GF, Clegg S, Allen BL

Abstract

Strains of Klebsiella pneumoniae are known to express two morphologically and functionally distinct filaments, the type 3 and the type 1 fimbriae. The gene (mrkD) encoding the adhesion of K. pneumoniae type 3 fimbriae was identified by transcomplementation analysis with the pap fimbrial gene cluster of Escherichia coli. The nucleotide sequence of the mrkD gene was determined. In addition, the determinant coding for the K. pneumoniae type 1 fimbrial adhesion was identified, and its nucleotide sequence was deduced. The predicted amino acid sequences of the K. pneumoniae adhesion proteins are compared, and similarities with the major fimbrial structural proteins (MrkA and FimA) are discussed.

MeSH Terms

Adhesins, Bacterial Adhesins, Escherichia coli Amino Acid Sequence Animals Bacterial Adhesion Bacterial Proteins/genetics Base Sequence Erythrocytes/immunology Escherichia coli/genetics,ultrastructure Fimbriae Proteins Fimbriae, Bacterial/physiology,ultrastructure Genes Genes, Bacterial Genetic Complementation Test Guinea Pigs Hemagglutination Humans Klebsiella pneumoniae/genetics,physiology Molecular Sequence Data Plasmids Restriction Mapping Sequence Homology, Nucleic Acid

Chemicals

Adhesins, Bacterial Adhesins, Escherichia coli Bacterial Proteins Klebsiella pneumoniae type 3 fimbrial adhesin fimH protein, E coli Fimbriae Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gerlach G F

Department of Microbiology, College of Medicine, University of Iowa, Iowa City 52242.

Clegg S

Allen B L

References (22)

22 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.171.3.1262-1270.1989

Published

1989-03-00

Pages

1262-70

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC209739

Subset

Grants

NIGMS NIH HHS · GM07337 · United States

NCRR NIH HHS · P41RR01685 · United States

Databases

GENBANK

M24536, M24564

External Links

PubMed source DOI: 10.1128/jb.171.3.1262-1270.1989 PMC Full Text View journal details

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Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae.

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