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PMID: 2566996 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Loss of heterozygosity for the short arm of chromosome 1 in human neuroblastomas: correlation with N-myc amplification.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 10 ·1989-05-00 ·Pages 3753-7

Fong CT, Dracopoli NC, White PS, Merrill PT, Griffith RC, Housman DE, Brodeur GM

Abstract

Partial monosomy of the short arm of chromosome 1 is the most consistent cytogenetic abnormality found in human neuroblastomas, but its overall frequency and significance are unclear. Using a panel of chromosome-1-specific DNA probes that identify restriction fragment length polymorphisms, we demonstrate that 13 of 47 human neuroblastomas (28%) have somatic loss of heterozygosity (LOH) at one or more loci on the distal short arm of chromosome 1. the chromosomal region that shows LOH most consistently is between 1p36.1 and 1p36.3; loss of a gene or genes in this region may be critical for the development or progression of neuroblastomas. The region of LOH in human neuroblastoma may resemble that described for pheochromocytoma, medullary thyroid carcinoma, and melanoma, which are also tumors of neural-crest origin. Although LOH for distal chromosome 1p can occur in early stages of neuroblastoma, the loss usually occurs in tumors of advanced clinical stages. LOH for the short arm of chromosome 1 correlates significantly with N-myc amplification, suggesting that these two genetic events are related. Indeed, these two lesions appear to characterize a genetically distinct subset of particularly aggressive neuroblastomas.

MeSH Terms
Chromosome Deletion Chromosome Mapping Chromosomes, Human, Pair 1 DNA Probes Gene Amplification Heterozygote Humans Neuroblastoma/genetics Polymorphism, Restriction Fragment Length Prognosis Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc
Chemicals
DNA Probes Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fong C T
Department of Pediatrics, Washington University School of Medicine, Saint Louis, MO 63110.
Dracopoli N C
White P S
Merrill P T
Griffith R C
Housman D E
Brodeur G M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.10.3753
Published
1989-05-00
Pages
3753-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287218
Subset
IM
Grants
NCI NIH HHS · CA01027 · United States
NCI NIH HHS · CA39771 · United States
NCI NIH HHS · CA44176 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.10.3753 PMC Full Text View journal details
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