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PMID: 2567284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Binding of Escherichia coli S fimbriae to cultured human endothelial cells.

Infection and immunity ·Vol. 57 ·No. 7 ·1989-07-00 ·Pages 2256-9

Parkkinen J, Ristimäki A, Westerlund B

Abstract

The binding of Escherichia coli adhesins to human umbilical vein endothelial cells was studied by a cell monolayer enzyme-linked immunosorbent assay. S fimbriae displayed a concentration-dependent and saturable binding to the endothelial cells which was mediated by their sialylgalactoside-specific lectin activity. P fimbriae exhibited only low binding, and type 1 fimbriae exhibited no binding to these cells.

MeSH Terms
Adhesins, Escherichia coli Bacterial Adhesion Bacterial Outer Membrane Proteins/analysis,physiology Blood Physiological Phenomena Cells, Cultured Endothelium, Vascular/analysis,microbiology Enzyme-Linked Immunosorbent Assay Escherichia coli/pathogenicity,physiology Fimbriae, Bacterial/analysis,physiology Humans Sialoglycoproteins/blood Umbilical Veins Virulence
Chemicals
Adhesins, Escherichia coli Bacterial Outer Membrane Proteins Sialoglycoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Parkkinen J
Department of Gynecology and Obstetrics, Helsinki University Central Hospital, Finland.
Ristimäki A
Westerlund B
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21 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.7.2256-2259.1989
Published
1989-07-00
Pages
2256-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313870
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.7.2256-2259.1989 PMC Full Text View journal details
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