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PMID: 2572562 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pseudomonas aeruginosa pili as ligands for nonopsonic phagocytosis by fibronectin-stimulated macrophages.

Infection and immunity ·Vol. 57 ·No. 12 ·1989-12-00 ·Pages 3841-5

Kelly NM, Kluftinger JL, Pasloske BL, Paranchych W, Hancock RE

Abstract

Fibronectin is capable of activating macrophages for enhanced nonopsonic phagocytosis of Pseudomonas aeruginosa grown in vivo in rats or mice or in vitro on nutrient agar plates. In this study it was determined that while fibronectin was able to significantly increase phagocytosis of organisms grown in static broth, uptake of agitated bacteria could not be promoted. Agitated P. aeruginosa cultures were proven to lack surface pili expression, as assessed by electron microscopic studies. A pilus-deficient pilA::Tn501 mutant of P. aeruginosa PAO was constructed by gene replacement techniques. Phagocytosis of this mutant could not be enhanced by fibronectin regardless of growth conditions. Furthermore, 60 micrograms of exogenously added Pseudomonas pili per ml was capable of abrogating the enhanced phagocytosis of the wild-type strain observed with fibronectin-stimulated macrophages. It is concluded that Pseudomonas pili were the bacterial ligands required for attachment to fibronectin-stimulated macrophages in the initial stages of nonopsonic phagocytosis.

MeSH Terms
Animals Bacterial Adhesion Bacterial Outer Membrane Proteins/genetics Blotting, Southern Cells, Cultured DNA Mutational Analysis DNA, Bacterial/genetics Fibronectins/pharmacology Fimbriae Proteins Fimbriae, Bacterial/physiology In Vitro Techniques Ligands Macrophages/immunology Mice Phagocytosis Pseudomonas aeruginosa/genetics,immunology
Chemicals
Bacterial Outer Membrane Proteins DNA, Bacterial Fibronectins Ligands Fimbriae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kelly N M
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Kluftinger J L
Pasloske B L
Paranchych W
Hancock R E
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.12.3841-3845.1989
Published
1989-12-00
Pages
3841-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259914
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.12.3841-3845.1989 PMC Full Text View journal details
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