Sequence and transcript analysis of the C. albicans URA3 gene encoding orotidine-5'-phosphate decarboxylase.

Current genetics ·Vol. 16 ·No. 3 ·1989-09-00 ·Pages 153-8

Losberger C, Ernst JF

Abstract

The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to a protein of 270 amino acids that shows homology to orotidine-5'-phosphate decarboxylases (ODCases) of other fungal species. The C. albicans ODCase is most closely related to the ODCases of the budding yeasts Kluyveromyces lactis and S. cerevisiae (74% and 71% homology, respectively). Most 5' ends of URA3 transcripts in the authentic host and in the heterologous host S. cerevisiae were found to be identical. These results demonstrate a close taxonomic relationship between non-pathogenic budding yeasts and C. albicans.

MeSH Terms

Amino Acid Sequence Base Sequence Candida albicans/enzymology,genetics Carboxy-Lyases/genetics Kluyveromyces/enzymology,genetics Molecular Sequence Data Orotidine-5'-Phosphate Decarboxylase/genetics Poly A/genetics RNA, Fungal/genetics RNA, Messenger/genetics Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid

Chemicals

RNA, Fungal RNA, Messenger Poly A Carboxy-Lyases Orotidine-5'-Phosphate Decarboxylase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Losberger C

Division of Molecular Biology, Glaxo Institute for Molecular Biology, Geneva, Switzerland.

Ernst J F

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23 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00391471

Published

1989-09-00

Pages

153-8

Language

English

Region

United States

NLM ID

8004904

Subset

Databases

GENBANK

X14198

External Links

PubMed source DOI: 10.1007/BF00391471 View journal details

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Sequence and transcript analysis of the C. albicans URA3 gene encoding orotidine-5'-phosphate decarboxylase.

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