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PMID: 2578198 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Hemagglutinin-neuraminidase glycoprotein as a determinant of pathogenicity in mumps virus hamster encephalitis: analysis of mutants selected with monoclonal antibodies.

Journal of virology ·Vol. 53 ·No. 1 ·1985-01-00 ·Pages 67-74

Löve A, Rydbeck R, Kristensson K, Orvell C, Norrby E

Abstract

With the aid of monoclonal antibodies directed against a specific site on the hemagglutinin-neuraminidase surface glycoprotein, four mutants of the Kilham neurotropic strain of mumps virus were isolated. All four mutants had increased neuraminidase activity. Two mutants (M10 and M12) lost their hemagglutination capacity with human O erythrocytes but retained their ability to agglutinate guinea pig erythrocytes at 4 degrees C. A third mutant (M11) showed a change in the molecular weight of the hemagglutinin-neuraminidase glycoprotein. These three mutants (M10, M11, and M12) showed unaltered capacity to infect tissue cultures and to cause encephalitis in newborn hamsters. A fourth mutant (M13) retained its hemagglutination activity and capacity to infect Vero cell cultures but showed significantly lower neurovirulence in the suckling hamster brain than did the parental Kilham strain and the other three mutants. Both the number of infected neurons and the amount of infectious virus in the brain was reduced. On the other hand, there were no apparent differences in the occurrence of viral antigen in ependymal cells, indicating a selective change in affinity for neurons in the brain. These results suggest that certain changes in the hemagglutinin-neuraminidase glycoprotein may lead to an alteration of the neuropathogenicity of the Kilham strain of mumps virus.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Antibody Complex Cell Line Chlorocebus aethiops Cricetinae Encephalitis/microbiology Epitopes/analysis HN Protein Hemadsorption Hemagglutination Tests Kidney Molecular Weight Mumps virus/pathogenicity Mutation Neuraminidase/analysis Species Specificity Viral Proteins/analysis,genetics
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Epitopes HN Protein Viral Proteins Neuraminidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Löve A
Rydbeck R
Kristensson K
Orvell C
Norrby E
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48 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.53.1.67-74.1985
Published
1985-01-00
Pages
67-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254979
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.53.1.67-74.1985 PMC Full Text View journal details
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