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PMID: 2579377 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Plasmid pT181 replication is regulated by two countertranscripts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 3 ·1985-02-00 ·Pages 638-42

Kumar CC, Novick RP

Abstract

A transcription map of the replication control region of the Staphylococcus aureus plasmid pT181 has been constructed. Two major leftward transcripts, RNA III and RNA IV, start at positions 339 and 413, respectively. These two RNAs can serve as mRNAs for a plasmid-specific replication protein RepC. Two short rightward transcripts, RNA I and RNA II, approximately 85 and 150 nucleotides long, respectively, start at position 246. These rightward transcripts (referred to as countertranscripts) do not appear to be translated but act directly as negative regulators of plasmid replication, probably by interfering with translation of the RepC mRNAs. There is no significant base sequence homology among the countertranscripts of pT181, ColE1, and R1/NR1/R6-5, suggesting that the structural parallelism has risen by convergent molecular evolution.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Replication Endonucleases/metabolism Nucleic Acid Conformation Plasmids RNA/biosynthesis Single-Strand Specific DNA and RNA Endonucleases Staphylococcus aureus/genetics Transcription, Genetic
Chemicals
RNA Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kumar C C
Novick R P
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.3.638
Published
1985-02-00
Pages
638-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397100
Subset
IM
Databases
GENBANK
M10415
External Links
PubMed source DOI: 10.1073/pnas.82.3.638 PMC Full Text View journal details
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