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PMID: 2581127 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of duplicated human c-src genes located on chromosomes 1 and 20.

Molecular and cellular biology ·Vol. 5 ·No. 4 ·1985-04-00 ·Pages 831-8

Parker RC, Mardon G, Lebo RV, Varmus HE, Bishop JM

Abstract

The oncogene (v-src) of Rous sarcoma virus apparently arose by transduction of the chicken gene known as c-src(chicken). We isolated DNA fragments representative of two src-related loci from recombinant DNA bacteriophage libraries of the human genome. One of these loci, c-src1(human), appeared to direct the synthesis of a 5-kilobase polyadenylated RNA that presumably encodes pp60c-src(human). Probes specific for the other locus, c-src2(human), did not hybridize to polyadenylated RNA prepared from a variety of human cell lines. Partial nucleotide sequence determinations of the loci demonstrated that c-src1(human) is highly related to chicken c-src and that c-src2(human) is slightly more divergent. The sequences imply that the final two coding exons of each human locus are identical in length to those of chicken c-src and that the location of an amber stop codon is unchanged in all three loci. c-src1(human) has been mapped to chromosome 20, and the second locus is located on chromosome 1. We conclude that c-src1(human) is the analog of c-src(chicken) and that the duplicated locus, c-src2(human), may also be expressed.

MeSH Terms
Animals Base Sequence Chickens Chromosome Mapping Chromosomes, Human, 1-3 Chromosomes, Human, 19-20 Cloning, Molecular Gene Expression Regulation Genes Humans Oncogenes Phosphoproteins/genetics Protein Kinases/genetics Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Chemicals
Phosphoproteins Protein Kinases Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Parker R C
Mardon G
Lebo R V
Varmus H E
Bishop J M
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.4.831-838.1985
Published
1985-04-00
Pages
831-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366788
Subset
IM
Grants
NCI NIH HHS · CA 12705 · United States
NCRR NIH HHS · SO7 RR05355 · United States
Databases
GENBANK
K03216, K03217, K03218, K03219
External Links
PubMed source DOI: 10.1128/mcb.5.4.831-838.1985 PMC Full Text View journal details
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