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PMID: 2582237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells.

Molecular and cellular biology ·Vol. 5 ·No. 5 ·1985-05-00 ·Pages 1073-83

Johnson PJ, Coussens PM, Danko AV, Shalloway D

Abstract

NIH 3T3 cells were transfected with plasmids containing Moloney murine leukemia virus long terminal repeats and either chicken c-src or v-src genes. In contrast with the effects observed after transfection with plasmids containing c-src and avian retrovirus or simian virus 40 promoter-enhancers (H. Hanafusa, H. Iba, T. Takeya, and F. R. Cross, p. 1-8, in G. F. Vande Woude, A. J. Levine, W. C. Topp, and J. D. Watson, ed., Cancer Cells, vol. 2, 1984; H. Iba, T. Takeya, F. R. Cross, T. Hanafusa, and H. Hanafusa, Proc. Natl. Acad. Sci. U.S.A. 81:4424-4428, 1984; R. C. Parker, R. Swanstrom, H. E. Varmus, and J. M. Bishop, p. 19-26, in G. F. Vande Woude et al., ed., Cancer Cells, vol. 2, 1984; R. C. Parker, H. E. Varmus, and J. M. Bishop, Cell 37:131-139, 1984; D. Shalloway, P. M. Coussens, and P. Yaciuk, p. 9-17, in G. F. Vande Woude et al., ed., Cancer Cells, vol. 2, 1984; D. Shalloway, P. M. Coussens, and P. Yaciuk, Proc. Natl. Acad. Sci. U.S.A. 81:7071-7075; and K. C. Wilhelmsen, W. G. Tarpley, and H. M. Temin, p. 303-308, in G. F. Vande Woude et al., ed., Cancer Cells, vol. 2, 1984), we found that both types of Moloney murine leukemia virus long terminal repeat-src expression plasmids induced focus formation, although c-src induced only 1% as many foci as v-src. The focus-selected c-src overexpressed cells had altered morphology and limited growth in soft agarose but were not tumorigenic in vivo. Cleveland digests, comparative in vitro kinase assays, secondary transfections, and immunoprecipitations indicated that focus formation was caused by rare transfection events that resulted in very high-level pp60c-src expression rather than by mutations of the transfected c-src genes. These results suggest that pp60v-src induced transformation is not a completely spurious activity which is unrelated to the function of pp60c-src but that it represents a perturbation of already existent molecular control processes involving pp60c-src.

MeSH Terms
Animals Cell Division Cell Transformation, Neoplastic/pathology Cells, Cultured Gene Expression Regulation Mice Molecular Weight Neoplasms, Experimental/pathology Oncogenes Peptide Fragments/analysis Phosphoproteins/genetics,physiology Plasmids Protein Kinases/genetics,metabolism Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src) Transfection
Chemicals
Peptide Fragments Phosphoproteins Protein Kinases Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Johnson P J
Coussens P M
Danko A V
Shalloway D
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27 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.5.1073-1083.1985
Published
1985-05-00
Pages
1073-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366824
Subset
IM
Grants
NCI NIH HHS · CA32317 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.5.1073-1083.1985 PMC Full Text View journal details
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