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PMID: 2583088 Published · ppublish English Journal Article

Cytoplasmic domains determine signal specificity, cellular routing characteristics and influence ligand binding of epidermal growth factor and insulin receptors.

The EMBO journal ·Vol. 8 ·No. 10 ·1989-10-00 ·Pages 2943-54

Riedel H, Dull TJ, Honegger AM, Schlessinger J, Ullrich A

Abstract

The cell surface receptors for insulin and epidermal growth factor (EGF) both employ a tyrosine-specific protein kinase activity to fulfil their distinct biological roles. To identify the structural domains responsible for various receptor activities, we have generated chimeric receptor polypeptides consisting of major EGF and insulin receptor structural domains and examined their biochemical properties and cellular signalling activities. The EGF-insulin receptor hybrids are properly synthesized and transported to the cell surface, where they form binding competent structures that are defined by the origin of their extracellular domains. While their ligand binding affinities are altered, we find that these chimeric receptors are fully functional in transmitting signals across the plasma membrane and into the cell. Thus, EGF receptor and insulin receptor cytoplasmic domain signalling capabilities are independent of their new heterotetrameric or monomeric environments respectively. Furthermore, the cytoplasmic domains carry the structural determinants that define kinase specificity, mitogenic and transforming potential, and receptor routing.

MeSH Terms
Animals Binding Sites Cell Division Cell Line Cell Membrane/metabolism Cell Transformation, Neoplastic Chimera Cytoplasm/metabolism DNA/metabolism ErbB Receptors/metabolism Fibroblasts Gene Expression Regulation Humans Ligands Mice Phosphorylation Receptor, Insulin/metabolism Signal Transduction Tetradecanoylphorbol Acetate/pharmacology Transfection
Chemicals
Ligands DNA ErbB Receptors Receptor, Insulin Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Riedel H
Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080.
Dull T J
Honegger A M
Schlessinger J
Ullrich A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-10-00
Pages
2943-54
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401363
Subset
IM
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