Home LiteratureArticle Details
PMID: 2584931 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Mac-2 antigen is a galactose-specific lectin that binds IgE.

The Journal of experimental medicine ·Vol. 170 ·No. 6 ·1989-12-01 ·Pages 1959-72

Cherayil BJ, Weiner SJ, Pillai S

Abstract

A cDNA encoding the Mac-2 antigen, a surface marker highly expressed by thioglycollate-elicited macrophages, has been cloned by immunoscreening of a lambda gt11-P388D1 expression library. The nucleotide sequence of the cDNA is identical to that of carbohydrate-binding protein 35, a galactose-specific lectin found in fibroblasts and highly homologous to a rat IgE-binding protein from basophilic leukemia cells. The in vitro synthesized Mac-2 protein displayed the expected carbohydrate- and IgE-binding properties. By pulse-chase analysis and subcellular fractionation studies, the Mac-2 protein was found in the cytosol but was also seen to accumulate in the extracellular medium. The latter finding was surprising in view of the fact that the cDNA did not encode a signal peptide or transmembrane domain. An alternatively spliced cDNA with the potential to encode a NH2 terminally extended Mac-2 protein with a stretch of hydrophobic amino acids at its NH2 terminus was also found, but it is not clear whether it is the source of the extracellular Mac-2. Possible functions for the Mac-2 protein based on its lectin- and IgE-binding properties are discussed.

MeSH Terms
Animals Antigens, Differentiation/genetics,physiology Base Sequence Carrier Proteins/genetics Cloning, Molecular DNA/analysis Galactose/metabolism Galectin 3 Galectins Hemagglutinins/genetics,physiology Immunoglobulin E/metabolism Macrophages/immunology Mice Mice, Inbred BALB C Mice, Inbred DBA Molecular Sequence Data
Chemicals
Antigens, Differentiation Carrier Proteins Galectin 3 Galectins Hemagglutinins Immunoglobulin E DNA Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cherayil B J
Molecular Immunology Laboratory, Cancer Center of the Massachusetts General Hospital, Boston, Massachusetts.
Weiner S J
Pillai S
References (41)
41 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Human basic fibroblast growth factor gene encodes four polypeptides: three initiate translation from non-AUG codons.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):3978-81 PMID: 2726761
  3. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
    Eur J Immunol. 1979 Apr;9(4):301-6 PMID: 89034
  4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  5. Mac-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies.
    J Immunol. 1982 Mar;128(3):1221-8 PMID: 6173426
  6. endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4540-4 PMID: 6812050
  7. Spontaneous in vitro occurrence and long-term culture of murine B lymphoblast cell lines.
    J Immunol. 1983 May;130(5):2113-6 PMID: 6339621
  8. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.
    Am J Pathol. 1983 Apr;111(1):112-24 PMID: 6340516
  9. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  10. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  11. A minimal intron length but no specific internal sequence is required for splicing the large rabbit beta-globin intron.
    Cell. 1984 Jul;37(3):915-25 PMID: 6204770
  12. Macrophage complement and lectin-like receptors bind Leishmania in the absence of serum.
    J Exp Med. 1985 Jul 1;162(1):324-31 PMID: 3891904
  13. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.
    Nature. 1985 Jun 20-26;315(6021):641-7 PMID: 2989698
  14. The Leishmania receptor for macrophages is a lipid-containing glycoconjugate.
    EMBO J. 1985 Feb;4(2):329-36 PMID: 4018028
  15. Galactose-specific receptors on liver cells. II. Characterization of the purified receptor from macrophages reveals no structural relationship to the hepatocyte receptor.
    Biochim Biophys Acta. 1985 Oct 30;847(1):115-21 PMID: 2413897
  16. Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor.
    Science. 1986 Aug 1;233(4763):545-8 PMID: 2425435
  17. Molecular structure of human lymphocyte receptor for immunoglobulin E.
    Cell. 1986 Dec 5;47(5):657-65 PMID: 2877743
  18. Properties and distribution of a lectin-like hemagglutinin differentially expressed by murine stromal tissue macrophages.
    J Exp Med. 1986 Dec 1;164(6):1862-75 PMID: 3783087
  19. Murine macrophage cell lines can be ordered in a linear differentiation sequence.
    Differentiation. 1986;32(2):157-64 PMID: 3792703
  20. Quantitative immunocytochemical characterization of mononuclear phagocytes. I. Monoblasts, promonocytes, monocytes, and peritoneal and alveolar macrophages.
    Cell Immunol. 1987 Apr 1;105(2):374-85 PMID: 3568140
  21. Serum lectin with known structure activates complement through the classical pathway.
    J Biol Chem. 1987 Jun 5;262(16):7451-4 PMID: 3584121
  22. Endogenous lectins from cultured cells: nuclear localization of carbohydrate-binding protein 35 in proliferating 3T3 fibroblasts.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6452-6 PMID: 3306680
  23. Isolation of RNA using guanidinium salts.
    Methods Enzymol. 1987;152:219-27 PMID: 2443794
  24. An IgE-binding protein with a distinctive repetitive sequence and homology with an IgG receptor.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6859-63 PMID: 2958848
  25. Lipophosphoglycan of Leishmania major that vaccinates against cutaneous leishmaniasis contains an alkylglycerophosphoinositol lipid anchor.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8941-5 PMID: 3480520
  26. Lambda gt 11: gene isolation with antibody probes and other applications.
    Methods Enzymol. 1987;154:107-28 PMID: 2448583
  27. A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas.
    Cell. 1988 Jan 29;52(2):185-95 PMID: 3277717
  28. A human mannose-binding protein is an acute-phase reactant that shares sequence homology with other vertebrate lectins.
    J Exp Med. 1988 Mar 1;167(3):1034-46 PMID: 2450948
  29. Carbohydrate binding protein 35. Complementary DNA sequence reveals homology with proteins of the heterogeneous nuclear RNP.
    J Biol Chem. 1988 May 5;263(13):6009-11 PMID: 3360772
  30. Molecular cloning and sequencing of a cDNA for a carbohydrate binding receptor unique to rat Kupffer cells.
    J Biol Chem. 1988 Jun 5;263(16):7487-92 PMID: 2836387
  31. IgE-binding factors and regulation of the IgE antibody response.
    Annu Rev Immunol. 1988;6:513-34 PMID: 3289576
  32. Two distinct classes of carbohydrate-recognition domains in animal lectins.
    J Biol Chem. 1988 Jul 15;263(20):9557-60 PMID: 3290208
  33. Protein translocation across membranes.
    Science. 1988 Sep 9;241(4871):1307-13 PMID: 2842866
  34. IgE-binding protein. Subcellular location and gene expression in many murine tissues and cells.
    J Immunol. 1988 Oct 15;141(8):2801-6 PMID: 3139767
  35. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 PMID: 2461560
  36. A human serum mannose-binding protein inhibits in vitro infection by the human immunodeficiency virus.
    J Exp Med. 1989 Jan 1;169(1):185-96 PMID: 2909656
  37. Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain.
    Science. 1989 Jan 13;243(4888):217-20 PMID: 2463672
  38. Adhesion molecules controlling lymphocyte migration.
    Cell. 1989 Mar 24;56(6):907-10 PMID: 2647304
  39. Translation initiation at non-AUG triplets in mammalian cells.
    J Biol Chem. 1989 Mar 25;264(9):5031-5 PMID: 2538469
  40. The human mannose-binding protein functions as an opsonin.
    J Exp Med. 1989 May 1;169(5):1733-45 PMID: 2469767
  41. Isolation of antibodies to protein hormones by bioaffinity chromatography on divinylsulfonyl sepharose.
    Biochem Biophys Res Commun. 1976 Mar 8;69(1):190-6 PMID: 1259756
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.170.6.1959
Published
1989-12-01
Pages
1959-72
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189532
Subset
IM
Grants
NIAID NIH HHS · AI-27835 · United States
Databases
GENBANK
X16834
External Links
PubMed source DOI: 10.1084/jem.170.6.1959 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2