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PMID: 2591369 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 6000 kb segment of chromosome 1 is conserved in human and mouse.

The EMBO journal ·Vol. 8 ·No. 13 ·1989-12-20 ·Pages 4073-80

Kingsmore SF, Watson ML, Howard TA, Seldin MF

Abstract

A murine linkage map generated from analyses of 428 meiotic events in an interspecific cross and pulsed field gel electrophoresis allowed examination of the genomic organization of a 6000 kb segment of mouse and human chromosome 1. Analysis of five genes within this syntenic segment of both species revealed striking conservation of gene order, intergenic distance and, to a lesser extent, CpG dinucleotides. In the mouse, meiotic crossover events were not evenly distributed; a hot spot for meiotic recombination was coincident with a CpG-island. These studies provide a practical approach to aid physical mapping of the human genome and a model for determining the molecular principles that govern meiotic recombination. In addition, these findings demonstrate profound conservation of genomic organization over mammalian evolution.

MeSH Terms
Animals Blotting, Southern Chromosome Mapping Chromosomes, Human, Pair 1 Crosses, Genetic DNA Probes Female Genes Hominidae/genetics Humans Male Mice Mice, Inbred C3H/genetics Muridae/genetics Nucleic Acid Hybridization Recombination, Genetic Restriction Mapping
Chemicals
DNA Probes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kingsmore S F
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Watson M L
Howard T A
Seldin M F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-12-20
Pages
4073-80
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401584
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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