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PMID: 2592417 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antibodies to the L1 adhesion molecule inhibit Schwann cell ensheathment of neurons in vitro.

The Journal of cell biology ·Vol. 109 ·No. 6 Pt 1 ·1989-12-00 ·Pages 3095-103

Seilheimer B, Persohn E, Schachner M

Abstract

To investigate whether neural adhesion molecules are involved in neuron-induced Schwann cell differentiation, cocultures of pure dorsal root ganglion neurons, and Schwann cells were maintained in the presence of antibodies to evaluate possible perturbing effects. Several parameters characteristic of differentiating Schwann cells were studied, such as transition of spindle-shaped to flattened, i.e., more epithelioid morphology, association with neuronal cell bodies, ensheathment of neurites, production of basal lamina and collagen fibrils, and expression of the myelin associated glycoprotein (MAG). A complete ablation of Schwann cell differentiation in all features studied was seen with antibodies to the neural adhesion molecule L1. Antibodies to N-CAM did not reduce the association of Schwann cells with neurites but abolished the interdigitation of Schwann cell processes into neurite bundles, while leaving the other parameters studied unaffected. Fab fragments of antibodies to J1, MAG, and mouse liver membranes did not interfere with the manifestation of any of these parameters. None of the antibodies changed incorporation of [3H]thymidine into Schwann cells.

MeSH Terms
Animals Antibodies Cell Adhesion Cell Adhesion Molecules, Neuronal/immunology,physiology Cells, Cultured DNA Replication Ganglia, Spinal/physiology Immunoglobulin Fab Fragments Immunoglobulin G Mice Mice, Inbred Strains Microscopy, Electron Neurons/cytology,physiology,ultrastructure Schwann Cells/cytology,physiology,ultrastructure Sciatic Nerve/physiology Thymidine/metabolism
Chemicals
Antibodies Cell Adhesion Molecules, Neuronal Immunoglobulin Fab Fragments Immunoglobulin G Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Seilheimer B
Department of Neurobiology, University of Heidelberg, Federal Republic of Germany.
Persohn E
Schachner M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.6.3095
Published
1989-12-00
Pages
3095-103
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115926
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.109.6.3095 PMC Full Text View journal details
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