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PMID: 2592571 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monocyte-chemotactic activity of defensins from human neutrophils.

The Journal of clinical investigation ·Vol. 84 ·No. 6 ·1989-12-00 ·Pages 2017-20

Territo MC, Ganz T, Selsted ME, Lehrer R

Abstract

We investigated the monocyte-chemotactic activity of fractionated extracts of human neutrophil granules. Monocyte-chemotactic activity was found predominantly in the defensin-containing fraction of the neutrophil granules. Purified preparations of each of the three human defensins (HNP-1, HNP-2, HNP-3) were then tested. HNP-1 demonstrated significant chemotactic activity for monocytes: Peak activity was seen at HNP-1 concentrations of 5 X 10(-9) M and was 49 +/- 20% (mean +/- SE, n = 9) of that elicited by 10(-8) M FMLP. HNP-2 (peak activity at 5 X 10(-9) M) was somewhat less active, yielding 19 +/- 10% (n = 11). HNP-3 failed to demonstrate chemotactic activity. Checkerboard analysis of monocyte response to HNP-1 and HNP-2 confirmed that their activity was chemotactic rather than chemokinetic. Neutrophils demonstrated a low level of response to defensins but this reaction was primarily chemokinetic. Defensins may play a role in the recruitment of monocytes by neutrophils into inflammatory sites.

MeSH Terms
Blood Proteins/isolation & purification,pharmacology Chemotaxis, Leukocyte/drug effects Cytoplasmic Granules/analysis Humans Monocytes/physiology N-Formylmethionine Leucyl-Phenylalanine/pharmacology Neutrophils/ultrastructure alpha-Defensins
Chemicals
Blood Proteins alpha-Defensins human neutrophil peptide 1 N-Formylmethionine Leucyl-Phenylalanine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Territo M C
Department of Medicine, University of California, Los Angeles 90024.
Ganz T
Selsted M E
Lehrer R
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114394
Published
1989-12-00
Pages
2017-20
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC304087
Subset
IM
Grants
NIAID NIH HHS · AI-22839 · United States
NIAID NIH HHS · AI-25693 · United States
NHLBI NIH HHS · HL-35640 · United States
External Links
PubMed source DOI: 10.1172/JCI114394 PMC Full Text View journal details
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