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PMID: 25926440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?

Biology of reproduction ·Vol. 92 ·No. 6 ·2015-06-00 ·Pages 144

Maillo V, Gaora PÓ, Forde N, Besenfelder U, Havlicek V, Burns GW, Spencer TE, Gutierrez-Adan A, Lonergan P, Rizos D

Abstract

This study examined the effect of the presence of single or multiple embryos on the transcriptome of the bovine oviduct. In experiment 1, cyclic (nonbred, n = 6) and pregnant (artificially inseminated, n = 11) heifers were slaughtered on Day 3 after estrus, and the ampulla and isthmic regions of the oviduct ipsilateral to the corpus luteum were separately flushed. Oviductal epithelial cells from the isthmus region, in which all oocytes/embryos were located, were snap-frozen for microarray analysis. In experiment 2, heifers were divided into cyclic (nonbred, n = 6) or pregnant (multiple embryo transfer, n = 10) groups. In vitro-produced presumptive zygotes were transferred endoscopically to the ipsilateral oviduct on Day 1.5 postestrus (n = 50 zygotes/heifer). Heifers were slaughtered on Day 3, and oviductal isthmus epithelial cells were recovered for RNA sequencing. Microarray analysis in experiment 1 failed to detect any difference in the transcriptome of the oviductal isthmus induced by the presence of a single embryo. In experiment 2, following multiple embryo transfer, RNA sequencing revealed 278 differentially expressed genes, of which 123 were up-regulated and 155 were down-regulated in pregnant heifers. Most of the down-regulated genes were related to immune function. In conclusion, the presence of multiple embryos in the oviduct resulted in the detection of differentially expressed genes in the oviductal isthmus; failure to detect changes in the oviduct transcriptome in the presence of a single embryo may be due to the effect being local and undetectable under the conditions of this study.

Keywords
RNA-Seq bovine embryo-maternal interaction microarray oviduct
MeSH Terms
Animals Cattle Embryo, Mammalian/metabolism,physiology Female Gene Expression Profiling Gene Expression Regulation Oviducts/metabolism,physiology Pregnancy Tissue Array Analysis Transcriptome
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Maillo Veronica
Departamento de Reproducción Animal, INIA, Madrid, Spain.
Gaora Peadar Ó
School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin, Ireland.
Forde Niamh
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
Besenfelder Urban
Reproduction Centre-Wieselburg, University of Veterinary Medicine Vienna, Vienna, Austria.
Havlicek Vitezslav
Reproduction Centre-Wieselburg, University of Veterinary Medicine Vienna, Vienna, Austria.
Burns Gregory W
Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, Washington.
Spencer Thomas E
Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, Washington.
Gutierrez-Adan Alfonso
Departamento de Reproducción Animal, INIA, Madrid, Spain.
Lonergan Patrick
School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
Rizos Dimitrios
Departamento de Reproducción Animal, INIA, Madrid, Spain [email protected].
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Article Info
Journal
Biology of reproduction
Abbr.
Biol Reprod
ISSN
1529-7268
DOI
10.1095/biolreprod.115.127969
Email
[email protected]
Published
2015-06-00
Epub
2015-00-29
Pages
144
Language
English
Region
United States
NLM ID
0207224
PMCID
PMC6366479
Subset
IM
Grants
NICHD NIH HHS · R01 HD072898 · United States
External Links
PubMed source DOI: 10.1095/biolreprod.115.127969 PMC Full Text View journal details
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