Electroporation induced transformation of Bacteroides ruminicola and Bacteroides uniformis by plasmid DNA.

FEMS microbiology letters ·Vol. 52 ·No. 1-2 ·1989-10-01 ·Pages 101-4

Thomson AM, Flint HJ

Abstract

Frequencies of greater than 10(5) transformants per microgram DNA were achieved in Bacteroides ruminicola F101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid pRRI4. Similar procedures gave frequences of 10(6) erythromycin resistant transformants per microgram DNA with the shuttle plasmid pDP1 (19 kbp) in Bacteroides uniformis. Transformation of B. uniformis occurred at a far lower frequency (10(3) micrograms) when pDP1 DNA was derived from E. coli rather than B. uniformis.

MeSH Terms

Bacteroides/genetics,growth & development DNA, Bacterial/genetics Electrochemistry Plasmids Transformation, Bacterial

Chemicals

DNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Thomson A M

Rowett Research Institute, Bucksburn, Aberdeen, U.K.

Flint H J

Article Info

Journal

FEMS microbiology letters

Abbr.

FEMS Microbiol Lett

ISSN

0378-1097

DOI

10.1016/0378-1097(89)90178-x

Published

1989-10-01

Pages

101-4

Language

English

Region

England

NLM ID

7705721

Subset

External Links

PubMed source DOI: 10.1016/0378-1097(89)90178-x View journal details

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Electroporation induced transformation of Bacteroides ruminicola and Bacteroides uniformis by plasmid DNA.

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